| Literature DB >> 3913395 |
L Meile, T Leisinger, J N Reeve.
Abstract
Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.Entities:
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Year: 1985 PMID: 3913395 DOI: 10.1007/bf00411245
Source DB: PubMed Journal: Arch Microbiol ISSN: 0302-8933 Impact factor: 2.552