Literature DB >> 3913331

Radioiodination of a photoactivatable heterobifunctional reagent.

I Ji, J Shin, T H Ji.   

Abstract

The N-hydroxysuccinimide ester of 4-azidosalicylic acid, a photoactivable heterobifunctional reagent, can be radioiodinated. The low efficiency (3%) of the radioiodination by a previously published method (I. Ji and T. H. Ji, 1982, Anal. Biochem. 121, 286-289) has been increased to 63% by substituting the solvent, acetone, with others such as aqueous acetonitrile, dimethylformamide, or dimethyl sulfoxide. The resulting 125I reagent was used for derivatizing human choriogonadotropin. The radioactive hormone derivative was crosslinked to the alpha beta dimer upon photolysis.

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Year:  1985        PMID： 3913331     DOI： 10.1016/0003-2697(85)90186-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Photoaffinity labeling of the multidrug-resistance-related P-glycoprotein with photoactive analogs of verapamil.

Authors:  A R Safa
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

2.  Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (phospho) lipase family.

Authors:  K Bhardwaj; A Raju; R Rajasekharan
Journal:  Plant Physiol       Date:  2001-12       Impact factor: 8.340

3.  The Drosophila acetylcholine receptor subunit D alpha5 is part of an alpha-bungarotoxin binding acetylcholine receptor.

Authors:  Peipei Wu; Dongdong Ma; Marek Pierzchala; Jun Wu; Lee-Chuan Yang; Xiaoping Mai; Xiaoying Chang; Thomas Schmidt-Glenewinkel
Journal:  J Biol Chem       Date:  2005-03-21       Impact factor: 5.157

4.  Photoaffinity Labeling of Developing Jojoba Seed Microsomal Membranes with a Photoreactive Analog of Acyl-Coenzyme A (Acyl-CoA) (Identification of a Putative Acyl-CoA:Fatty Alcohol Acyltransferase.

Authors:  J. M. Shockey; R. Rajasekharan; J. D. Kemp
Journal:  Plant Physiol       Date:  1995-01       Impact factor: 8.340

5.  Regulation of the skeletal sarcoplasmic reticulum Ca(2+)-ATPase by phospholamban and negatively charged phospholipids in reconstituted phospholipid vesicles.

Authors:  G Szymańska; H W Kim; J Cuppoletti; E G Kranias
Journal:  Mol Cell Biochem       Date:  1992-09-08       Impact factor: 3.396

6.  Photolabelling of Salmonella typhimurium LT2 sialidase. Identification of a peptide with a predicted structural similarity to the active sites of influenza-virus sialidases.

Authors:  T G Warner; R Harris; R McDowell; E R Vimr
Journal:  Biochem J       Date:  1992-08-01       Impact factor: 3.857
  6 in total

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