Literature DB >> 3913327

Method of enzymatic determination of pyrroloquinoline quinone.

M Ameyama, M Nonobe, E Shinagawa, K Matsushita, O Adachi.   

Abstract

An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which D-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by EDTA treatment. Incubation of the EDTA-treated membrane with exogenous pyrroloquinoline quinone in the presence of magnesium ions gave a quantitative determination of pyrroloquinoline quinone by assaying the restored D-glucose dehydrogenase activity. This novel enzymatic method was confirmed to be highly reproducible up to 10 ng of pyrroloquinoline quinone and could be applied to a routine assay of pyrroloquinoline quinone.

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Year:  1985        PMID： 3913327     DOI： 10.1016/0003-2697(85)90174-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Reconstitution of pyrroloquinoline quinone-dependent D-glucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase.

Authors:  K Matsushita; M Nonobe; E Shinagawa; O Adachi; M Ameyama
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

2.  Kinetics and thermodynamics of activation of quinoprotein glucose dehydrogenase apoenzyme in vivo and catalytic activity of the activated enzyme in Escherichia coli cells.

Authors:  D Iswantini; K Kano; T Ikeda
Journal:  Biochem J       Date:  2000-09-15       Impact factor: 3.857

3.  Purification of a marine bacterial glucose dehydrogenase from Cytophaga marinoflava and its application for measurement of 1,5-anhydro-D-glucitol.

Authors:  W Tsugawa; S Horiuchi; M Tanaka; H Wake; K Sode
Journal:  Appl Biochem Biotechnol       Date:  1996-03       Impact factor: 2.926

4.  Generation mechanism and purification of an inactive form convertible in vivo to the active form of quinoprotein alcohol dehydrogenase in Gluconobacter suboxydans.

Authors:  K Matsushita; T Yakushi; Y Takaki; H Toyama; O Adachi
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

5.  Three distinct quinoprotein alcohol dehydrogenases are expressed when Pseudomonas putida is grown on different alcohols.

Authors:  H Toyama; A Fujii; K Matsushita; E Shinagawa; M Ameyama; O Adachi
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

6.  Determination of pyrroloquinoline quinone by enzymatic and LC-MS/MS methods to clarify its levels in foods.

Authors:  Chikara Kato; Emiko Kawai; Naoki Shimizu; Tsuyoshi Mikekado; Fumiko Kimura; Teruo Miyazawa; Kiyotaka Nakagawa
Journal:  PLoS One       Date:  2018-12-21       Impact factor: 3.240

7.  Identification of transcriptional networks responding to pyrroloquinoline quinone dietary supplementation and their influence on thioredoxin expression, and the JAK/STAT and MAPK pathways.

Authors:  Eskouhie Tchaparian; Lisa Marshal; Gene Cutler; Kathryn Bauerly; Winyoo Chowanadisai; Michael Satre; Calliandra Harris; Robert B Rucker
Journal:  Biochem J       Date:  2010-08-01       Impact factor: 3.857
  7 in total

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