Literature DB >> 3910622

Immuno gold staining (IGS) for electron microscopical demonstration of glial fibrillary acidic (GFA) protein in LR white embedded tissue.

M B Gräber, G W Kreutzberg.   

Abstract

Post-embedding immunocytochemical staining methods using gold have so far failed to label intermediate filament antigens in situ in epon or araldite embedded tissue. We have now applied the post-embedding immuno gold staining (IGS) technique for LR White embedded tissue. Glial fibrillary acidic (GFA) protein immunoreactivity was clearly demonstrated electron microscopically on astrocytic filaments of rat cerebellum in situ.

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Year:  1985        PMID： 3910622     DOI： 10.1007/bf00492450

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  16 in total

1.  FIXATION OF THE CENTRAL NERVOUS SYSTEM FROM ELECTRON MICROSCOPY BY ALDEHYDE PERFUSION. I. PRESERVATION WITH ALDEHYDE PERFUSATES VERSUS DIRECT PERFUSION WITH OSMIUM TETROXIDE WITH SPECIAL REFERENCE TO MEMBRANES AND THE EXTRACELLULAR SPACE.

Authors:  U KARLSSON; R L SCHULTZ
Journal:  J Ultrastruct Res       Date:  1965-02

2.  An immunocolloid method for the electron microscope.

Authors:  W P Faulk; G M Taylor
Journal:  Immunochemistry       Date:  1971-11

3.  Dehydration and embedding temperatures affect the antigenic specificity of tubulin and immunolabeling by the protein A-colloidal gold technique.

Authors:  B L Armbruster; R M Garavito; E Kellenberger
Journal:  J Histochem Cytochem       Date:  1983-12       Impact factor: 2.479

4.  Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique.

Authors:  M Bendayan; M Zollinger
Journal:  J Histochem Cytochem       Date:  1983-01       Impact factor: 2.479

5.  Immunocytochemical localization of tubulin with colloidal gold in cilia of rabbit tracheal epithelial cultures.

Authors:  P C Moller; J P Chang; L R Partridge
Journal:  Tissue Cell       Date:  1983       Impact factor: 2.466

6.  Laminin in cultured mouse C1300 neuroblastoma cells: immunocytochemical localization by pre- and postembedding electron microscope procedures.

Authors:  P Liesi
Journal:  J Histochem Cytochem       Date:  1983-06       Impact factor: 2.479

7.  High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method.

Authors:  J De Mey; M Moeremans; G Geuens; R Nuydens; M De Brabander
Journal:  Cell Biol Int Rep       Date:  1981-09

8.  Ultrastructural localization of tubulin and actin in polyethylene glycol-embedded rat seminiferous epithelium by immunogold staining.

Authors:  J J Wolosewick; J De Mey; V Meininger
Journal:  Biol Cell       Date:  1983       Impact factor: 4.458

9.  Attachment of vimentin filaments to desmosomal plaques in human meningiomal cells and arachnoidal tissue.

Authors:  J Kartenbeck; K Schwechheimer; R Moll; W W Franke
Journal:  J Cell Biol       Date:  1984-03       Impact factor: 10.539

10.  Ultrastructural localization of glial fibrillary acidic protein in mouse cerebellum by immunoperoxidase labeling.

Authors:  M Schachner; E T Hedley-Whyte; D W Hsu; G Schoonmaker; A Bignami
Journal:  J Cell Biol       Date:  1977-10       Impact factor: 10.539
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  9 in total

1.  LR Gold embedding of nervous tissue for immunoelectron microscopy studies.

Authors:  A Migheli; A Attanasio; D Schiffer
Journal:  Histochemistry       Date:  1992

2.  Ultrastructural immunocytochemistry of glia cells. Double labeling studies using LR White embedding and colloidal gold.

Authors:  M Goto; R Meyermann; H Wekerle
Journal:  Histochemistry       Date:  1987

3.  Astrocyte gene expression in Creutzfeldt-Jakob disease.

Authors:  L Manuelidis; D M Tesin; T Sklaviadis; E E Manuelidis
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

4.  A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays.

Authors:  Abraham Rosas-Arellano; Juan B Villalobos-González; Lourdes Palma-Tirado; Felipe A Beltrán; Alfonso Cárabez-Trejo; Fanis Missirlis; Maite A Castro
Journal:  Histochem Cell Biol       Date:  2016-05-17       Impact factor: 4.304

5.  Use and abuse of LR White.

Authors:  G R Newman
Journal:  Histochem J       Date:  1987-02

6.  Cuprizone Administration Alters the Iron Metabolism in the Mouse Model of Multiple Sclerosis.

Authors:  E Varga; E Pandur; H Abrahám; A Horváth; P Ács; S Komoly; A Miseta; K Sipos
Journal:  Cell Mol Neurobiol       Date:  2018-02-20       Impact factor: 5.046

7.  Immunogold electron microscopic localization of glial fibrillary acidic protein (GFAP) in neurohypophyseal pituicytes and tanycytes of the Mongolian gerbil (Meriones unguiculatus).

Authors:  P Redecker
Journal:  Histochemistry       Date:  1989

8.  Ultrastructural characteristics of glial fibrillary acidic protein expression in epoxy resin-embedded human brain tumors.

Authors:  B Bozóky; T Krenács; Z Rázga; A Erdös
Journal:  Acta Neuropathol       Date:  1993       Impact factor: 17.088

9.  Golgi-like immunostaining of pituicytes and tanycytes positive for glial fibrillary acidic protein in the neurohypophysis of the Mongolian gerbil (Meriones unguiculatus).

Authors:  P Redecker
Journal:  Histochemistry       Date:  1987
  9 in total

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