Literature DB >> 3910042

Cloning and expression of tryptophan genes from Brevibacterium lactofermentum in Escherichia coli.

G del Real, A Aguilar, J F Martín.   

Abstract

A gene bank from the amino acid producer Brevibacterium lactofermentum has been prepared in Escherichia coli using pBR322 as vector. Four clones containing genetic information needed to complement mutations in A,B,C and D genes from E. coli have been isolated. The cloned fragments range between 4.3 kb (pULT61) and 7.9 kb (pULT62). All the four clones contain genetic information that complements trpB gene from E. coli. The cloned trpB gene is very stable and is maintained extrachromosomally in E. coli. It is expressed very efficiently showing high levels of tryptophan synthetase activity.

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Year:  1985        PMID： 3910042     DOI： 10.1016/0006-291x(85)91237-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

1.  A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function.

Authors:  A Pisabarro; M Malumbres; L M Mateos; J A Oguiza; J F Martín
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490

2.  Molecular cloning of the hom-thrC-thrB cluster from Bacillus sp. ULM1: expression of the thrC gene in Escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes.

Authors:  M Malumbres; L M Mateos; C Guerrero; J F Martín
Journal:  Folia Microbiol (Praha)       Date:  1995       Impact factor: 2.099

3.  Cloning and expression in Escherichia coli of the homoserine kinase (thrB) gene from Brevibacterium lactofermentum.

Authors:  L M Mateos; G del Real; A Aguilar; J F Martín
Journal:  Mol Gen Genet       Date:  1987-03

4.  Cloning of the trp gene cluster from a tryptophan-hyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence.

Authors:  D M Heery; L K Dunican
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

5.  General organization of the genes specifically involved in the diaminopimelate-lysine biosynthetic pathway of Corynebacterium glutamicum.

Authors:  P Yeh; A M Sicard; A J Sinskey
Journal:  Mol Gen Genet       Date:  1988-04

6.  Cloning of FAD synthetase gene from Corynebacterium ammoniagenes and its application to FAD and FMN production.

Authors:  T Hagihara; T Fujio; K Aisaka
Journal:  Appl Microbiol Biotechnol       Date:  1995-01       Impact factor: 4.813

7.  Cosmid cloning of five Zymomonas trp genes by complementation of Escherichia coli and Pseudomonas putida trp mutants.

Authors:  C K Eddy; O H Smith; K D Noel
Journal:  J Bacteriol       Date:  1988-07       Impact factor: 3.490

8.  Analysis and expression of the thrC gene of Brevibacterium lactofermentum and characterization of the encoded threonine synthase.

Authors:  M Malumbres; L M Mateos; M A Lumbreras; C Guerrero; J F Martín
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792
  8 in total

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