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Literature DB >> 3910040

Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the in vitro expression of the metB, metL and metJ genes.

R Shoeman, T Coleman, B Redfield, R C Greene, A A Smith, I Saint-Girons, N Brot, H Weissbach.

Abstract

The regulation of the expression of three Escherichia coli met genes, metB, which codes for cystathionine gamma-synthetase (EC 4.2.99.9), metL, which codes for aspartokinase II-homoserine dehydrogenase II (EC 2.7.2.4-EC 1.1.1.3) and metJ, which codes for the methionine regulon aporepressor, has been studied using highly purified DNA-directed in vitro protein synthesis systems. In a system where the entire gene product is synthesized, the expression of the metB and metL genes is specifically inhibited by MetJ protein (repressor protein) and S-adenosylmethionine (AdoMet). In a simplified system that measures the formation of the first dipeptide of the gene product (fMet-Ala for the metJ gene), MetJ protein and AdoMet partially repress (approximately 40-60%) metJ gene expression. Thus, the metJ gene can be partially autoregulated by its gene product.

Entities: Chemical Gene Species

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Year: 1985 PMID： 3910040 DOI： 10.1016/0006-291x(85)90965-9

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

14 in total

1. Structure-function studies on Escherichia coli MetR protein, a putative prokaryotic leucine zipper protein.

Authors: M E Maxon; J Wigboldus; N Brot; H Weissbach
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

2. Mutations affecting regulation of cobinamide biosynthesis in Salmonella typhimurium.

Authors: D I Andersson; J R Roth
Journal: J Bacteriol Date: 1989-12 Impact factor: 3.490

3. Characterization of two mutant metJ proteins with reduced, temperature-dependent capacity to regulate Escherichia coli K-12 met regulon elements.

Authors: G A Bala; C D Collier; M R Emmett; J R Johnson
Journal: J Bacteriol Date: 1989-07 Impact factor: 3.490

4. The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression.

Authors: C D Collier; J R Johnson
Journal: J Bacteriol Date: 1990-07 Impact factor: 3.490

Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the in vitro expression of the metB, metL and metJ genes.

1. Structure-function studies on Escherichia coli MetR protein, a putative prokaryotic leucine zipper protein.

2. Mutations affecting regulation of cobinamide biosynthesis in Salmonella typhimurium.

3. Characterization of two mutant metJ proteins with reduced, temperature-dependent capacity to regulate Escherichia coli K-12 met regulon elements.

4. The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression.

5. Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression.

6. Cloning and characterization of the genes for the two homocysteine transmethylases of Escherichia coli.

7. Two transsulfurylation pathways in Klebsiella pneumoniae.

8. Regulation of cobalamin biosynthetic operons in Salmonella typhimurium.

9. Complementation of a metK-deficient E. coli strain with heterologous AdoMet synthetase genes.

10. S-methylmethionine metabolism in Escherichia coli.