Linkage of loci encoding a kidney endothelial antigen and fumarate hydratase (Fh-1) in the rat.

In the rat a single locus, provisionally designated Eag-1, controls the expression of an antigen present on the endothelium of kidney peritubular capillaries and veins. We have examined the linkage relationship between Eag-1 and 10 polymorphic loci including hemoglobin b, fumarate hydratase, peptidase-3, urinary pepsinogen, seminal vesicle protein, glycerophosphate dehydrogenase, esterase-1, esterase-6, pinkeye, and hooded. Tissue samples from animals derived from (AUG X BN.1C)F1 X AUG and (AUG X BN.1C)F1 X BN.1C backcrosses were examined and a linkage association between Eag-1 and Fh-1 (EC 4.2.2.1) was detected. The linkage distance between Eag-1 and Fh-1 is 21 cM (chi 2 = 27.9; p = 0.00001) and this association defines the third locus in the tenth (X) linkage group of the rat.