Literature DB >> 3908222

Expression of a synthetic human growth hormone gene in yeast.

T Tokunaga, S Iwai, H Gomi, K Kodama, E Ohtsuka, M Ikehara, O Chisaka, K Matsubara.   

Abstract

A synthetic human growth hormone (hGH) gene was efficiently expressed under the control of the repressible acid phosphatase promoter in yeast (Saccharomyces cerevisiae). More than 10(6) molecules of hormone were formed per cell despite the fact that the gene was constructed with codon preference for Escherichia coli.

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Year:  1985        PMID： 3908222     DOI： 10.1016/0378-1119(85)90117-9

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  3 in total

1.  Synonymous codon usage in Bacillus subtilis reflects both translational selection and mutational biases.

Authors:  D C Shields; P M Sharp
Journal:  Nucleic Acids Res       Date:  1987-10-12       Impact factor: 16.971

2.  Cloning, expression, and purification of a synthetic human growth hormone in Escherichia coli using response surface methodology.

Authors:  Mozhdeh Zamani; Aydin Berenjian; Shiva Hemmati; Navid Nezafat; Mohammad Bagher Ghoshoon; Fatemeh Dabbagh; Milad Mohkam; Younes Ghasemi
Journal:  Mol Biotechnol       Date:  2015-03       Impact factor: 2.695

3.  Dual-Color Monitoring Overcomes the Limitations of Single Bioluminescent Reporters in Fast-Growing Microbes and Reveals Phase-Dependent Protein Productivity during the Metabolic Rhythms of Saccharomyces cerevisiae.

Authors:  Archana Krishnamoorthy; J Brian Robertson
Journal:  Appl Environ Microbiol       Date:  2015-07-10       Impact factor: 4.792
  3 in total

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