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Literature DB >> 3907558

An improved technique for the cultivation of Plasmodium falciparum in vitro without daily medium change.

Abstract

A simple method is described allowing growth of Plasmodium falciparum in suspension cultures with 1% outdated human erythrocytes without the need for medium change for three to four days. Depending on the initial parasitaemia, final parasitaemias of from 10% to 30% can be obtained. Growth is exponential and asynchronous with a mean multiplication rate of 7.7 +/- 1.0 (n = 5) -fold per 48-hour cycle. The method is suitable for cultivation of a wide range of laboratory strains of P. falciparum, as well as for primary isolations of clinical material.

Entities: Species

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Substances：
Culture Media

Year: 1985 PMID： 3907558 DOI： 10.1080/00034983.1985.11811935

Source DB: PubMed Journal: Ann Trop Med Parasitol ISSN： 0003-4983

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Cited

17 in total

1. Antiplasmodial activity of Artemisia annua plant cell cultures.

Authors: N K Tawfiq; L A Anderson; M F Roberts; J D Phillipson; D H Bray; D C Warhurst
Journal: Plant Cell Rep Date: 1989-10 Impact factor: 4.570

2. Programmed transcription of the var gene family, but not of stevor, in Plasmodium falciparum gametocytes.

Authors: Sarah Sharp; Thomas Lavstsen; Quinton L Fivelman; Maha Saeed; Louisa McRobert; Thomas J Templeton; Anja T R Jensen; David A Baker; Thor G Theander; Colin J Sutherland
Journal: Eukaryot Cell Date: 2006-08

3. Plants as sources of antimalarial drugs: in vitro antimalarial activities of some quassinoids.

Authors: M J O'Neill; D H Bray; P Boardman; J D Phillipson; D C Warhurst; W Peters; M Suffness
Journal: Antimicrob Agents Chemother Date: 1986-07 Impact factor: 5.191

4. In vivo and in vitro antiplasmodial activities of some plants traditionally used in Guatemala against malaria.

Authors: F F Franssen; L J Smeijsters; I Berger; B E Medinilla Aldana
Journal: Antimicrob Agents Chemother Date: 1997-07 Impact factor: 5.191

5. Molecular cloning of a Plasmodium falciparum gene interrupted by 15 introns encoding a functional primase 53 kDa subunit as demonstrated by expression in a baculovirus system.

Authors: S Prasartkaew; N M Zijlstra; P Wilairat; J P Overdulve; E de Vries
Journal: Nucleic Acids Res Date: 1996-10-15 Impact factor: 16.971

6. Simple, fast, and accurate fluorometric method to determine drug susceptibility of Plasmodium falciparum in 24-well suspension cultures.

Authors: L J Smeijsters; N M Zijlstra; F F Franssen; J P Overdulve
Journal: Antimicrob Agents Chemother Date: 1996-04 Impact factor: 5.191

An improved technique for the cultivation of Plasmodium falciparum in vitro without daily medium change.

1. Antiplasmodial activity of Artemisia annua plant cell cultures.

2. Programmed transcription of the var gene family, but not of stevor, in Plasmodium falciparum gametocytes.

3. Plants as sources of antimalarial drugs: in vitro antimalarial activities of some quassinoids.

4. In vivo and in vitro antiplasmodial activities of some plants traditionally used in Guatemala against malaria.

5. Molecular cloning of a Plasmodium falciparum gene interrupted by 15 introns encoding a functional primase 53 kDa subunit as demonstrated by expression in a baculovirus system.

6. Simple, fast, and accurate fluorometric method to determine drug susceptibility of Plasmodium falciparum in 24-well suspension cultures.

7. Plasmodium falciparum infection of the placenta affects newborn immune responses.

8. In vitro antiplasmodial, antiamoebic, and cytotoxic activities of a series of bisbenzylisoquinoline alkaloids.

9. Stage-dependent effects of analogs of gramicidin A on the growth of Plasmodium falciparum in vitro.

10. Antimalarial activity of a cis-terpenone.