| Literature DB >> 3907158 |
A Guillouzo, J M Begue, J P Campion, M N Gascoin, C Guguen-Guillouzo.
Abstract
Viable adult human hepatocytes were obtained in large yields by perfusion of the liver of kidney donors. The hepatocytes were cultured either alone or in association with rat-liver epithelial cells. In pure culture the survival of hepatocytes did not exceed two to three weeks, while in co-culture they survived for several weeks and better retained the specific liver functions of albumin secretion, cytochrome P-450 content and glucuronidation of drugs. Human hepatocytes, particularly when mixed with rat-liver epithelial cells, may provide a valuable tool for predicting the metabolic pathways and hepatotoxicity of new drugs in man.Entities:
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Year: 1985 PMID： 3907158 DOI： 10.3109/00498258509047421
Source DB: PubMed Journal: Xenobiotica ISSN： 0049-8254 Impact factor: 1.908