Literature DB >> 3906896

Systematic variation in DNA length yields highly ordered repressor-operator cocrystals.

S R Jordan, T V Whitcombe, J M Berg, C O Pabo.   

Abstract

Crystals have been grown that contain the operator-binding domain of the lambda repressor and the lambda operator site OL1. Crystallization conditions were tested with a set of DNA fragments, ranging in length from 17 to 23 base pairs. The best crystals were grown with a 20-base pair DNA fragment. These crystals have space-group symmetry P2I, with unit cell dimensions a = 37.1 A, b = 68.8 A, c = 56.8 A, and a beta angle of 91.5 degrees. They diffracted to at least 2.5 A resolution. High resolution data from these crystals should allow the direct determination of how a repressor recognizes its operator site.

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Year:  1985        PMID： 3906896     DOI： 10.1126/science.3906896

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  9 in total

1.  Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein.

Authors:  T D Tullius; B A Dombroski
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

Review 2.  Structural aspects of protein-DNA recognition.

Authors:  P S Freemont; A N Lane; M R Sanderson
Journal:  Biochem J       Date:  1991-08-15       Impact factor: 3.857

3.  lac repressor: crystallization of intact tetramer and its complexes with inducer and operator DNA.

Authors:  H C Pace; P Lu; M Lewis
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

4.  Iterative optimization of DNA duplexes for crystallization of SeqA-DNA complexes.

Authors:  Yu Seon Chung; Alba Guarné
Journal:  J Vis Exp       Date:  2012-11-01       Impact factor: 1.355

5.  Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals.

Authors:  Edward E Pryor; Daniel J Wozniak; Thomas Hollis
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-07-31

6.  Crystallization, dehydration and preliminary X-ray analysis of excisionase (Xis) proteins cooperatively bound to DNA.

Authors:  My D Sam; Mohamad A Abbani; Duilio Cascio; Reid C Johnson; Robert T Clubb
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-07-26

Review 7.  Use of the spliceosomal protein U1A to facilitate crystallization and structure determination of complex RNAs.

Authors:  Adrian R Ferré-D'Amaré
Journal:  Methods       Date:  2010-06-08       Impact factor: 3.608

Review 8.  An overview of biological macromolecule crystallization.

Authors:  Irene Russo Krauss; Antonello Merlino; Alessandro Vergara; Filomena Sica
Journal:  Int J Mol Sci       Date:  2013-05-31       Impact factor: 5.923

Review 9.  Engineering altered protein-DNA recognition specificity.

Authors:  Adam J Bogdanove; Andrew Bohm; Jeffrey C Miller; Richard D Morgan; Barry L Stoddard
Journal:  Nucleic Acids Res       Date:  2018-06-01       Impact factor: 16.971
  9 in total

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