Literature DB >> 3905722

Monoclonal antibody to human lysosomal alpha-glucosidase in immunocytochemistry: unexpected reactivity with cytoskeletal structures.

H J Sips, N De Jonge, H M Van Dongen, F C Ramaekers, A J Reuser.   

Abstract

A well-characterized monoclonal antibody against human lysosomal alpha-glucosidase has been used for the immunohistochemical localization of the enzyme in cultured human skin fibroblasts. Under conditions that are routinely used for the preparation of cells for immunocytochemistry, this monoclonal antibody does not react with acid alpha-glucosidase but in contrast with components of the cytoskeleton. Double-labelling experiments with the monoclonal antibody and rabbit anti-vimentin antiserum identified the cytoskeletal components as intermediate filaments. The implications of this observation for the use of monoclonal antibodies in immunocytochemistry in general are discussed.

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Year:  1985        PMID： 3905722     DOI： 10.1007/bf01417953

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  10 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

2.  Intermediate filaments.

Authors:  M Osborn; N Geisler; G Shaw; G Sharp; K Weber
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1982

3.  Association of microtubules and intermediate filaments in normal fibroblasts and its disruption upon transformation by a temperature-sensitive mutant of Rous sarcoma virus.

Authors:  E H Ball; S J Singer
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

4.  Use of a monoclonal antibody to distinguish between precursor and mature forms of human lysosomal alpha-glucosidase.

Authors:  R P Oude Elferink; A Strijland; I Surya; E M Brouwer-Kelder; M Kroos; J Hilkens; J Hilgers; A J Reuser; J M Tager
Journal:  Eur J Biochem       Date:  1984-03-15

5.  Biosynthesis of acid alpha-glucosidase in late-onset forms of glycogenosis type II (Pompe's disease).

Authors:  F Steckel; V Gieselmann; A Waheed; A Hasilik; K von Figura; R Oude Elferink; R Kalsbeek; J M Tager
Journal:  FEBS Lett       Date:  1982-12-13       Impact factor: 4.124

6.  Loss of antibody binding to prefixed cells: fixation parameters for immunocytochemistry.

Authors:  W Van Ewijk; P L Van Soest; A Verkerk; J F Jongkind
Journal:  Histochem J       Date:  1984-02

7.  Uptake and stability of human and bovine acid alpha-glucosidase in cultured fibroblasts and skeletal muscle cells from glycogenosis type II patients.

Authors:  A J Reuser; M A Kroos; N J Ponne; R A Wolterman; M C Loonen; H F Busch; W J Visser; P A Bolhuis
Journal:  Exp Cell Res       Date:  1984-11       Impact factor: 3.905

8.  Immunocytochemistry of lysosomal hydrolases and their precursor forms in normal and mutant human cells.

Authors:  J M Van Dongen; R A Barneveld; H J Geuze; H Galjaard
Journal:  Histochem J       Date:  1984-09

9.  Defects in synthesis, phosphorylation, and maturation of acid alpha-glucosidase in glycogenosis type II.

Authors:  A J Reuser; M Kroos; R P Oude Elferink; J M Tager
Journal:  J Biol Chem       Date:  1985-07-15       Impact factor: 5.157

Review 10.  Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview.

Authors:  F C Ramaekers; J J Puts; O Moesker; A Kant; A Huysmans; D Haag; P H Jap; C J Herman; G P Vooijs
Journal:  Histochem J       Date:  1983-07
  10 in total

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