Literature DB >> 390302

Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants.

P V Venkov.   

Abstract

Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.

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Year:  1979        PMID： 390302     DOI： 10.1007/bf00267862

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  9 in total

1.  Two forms of a mitochondrial endonuclease in Neurospora crassa.

Authors:  C E Martin; R P Wagner
Journal:  Can J Biochem       Date:  1975-07

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  The development patterns of lysosomal enzyme activities during Ca2+-induced sporangium formation in Achyla bisexualis. III. Ribonucleases.

Authors:  R B Sutherland; B M Schuerch; S F Ball; P A Horgen
Journal:  Arch Microbiol       Date:  1976-09-01       Impact factor: 2.552

4.  Saccharomyces cerevisiae: sorbitol-dependent fragile mutants.

Authors:  P V Venkov; A A Hadjiolov; E Battaner; D Schlessinger
Journal:  Biochem Biophys Res Commun       Date:  1974-02-04       Impact factor: 3.575

5.  Polyadenylate metabolism in the nuclei and cytoplasm of Saccharomyces cerevisiae.

Authors:  B Groner; S L Phillips
Journal:  J Biol Chem       Date:  1975-07-25       Impact factor: 5.157

6.  Ribosomal subunit entry into polysomes in yeast.

Authors:  N Petersen; C S McLaughlin; D P Nierlich
Journal:  Biochim Biophys Acta       Date:  1976-10-18

7.  Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA.

Authors:  J A Bantle; I H Maxwell; W E Hahn
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

8.  A simplified assay for RNase activity in crude tissue extracts.

Authors:  E Ambellan; V P Hollander
Journal:  Anal Biochem       Date:  1966-12       Impact factor: 3.365

9.  Macromolecule synthesis in yeast spheroplasts.

Authors:  H T Hutchison; L H Hartwell
Journal:  J Bacteriol       Date:  1967-11       Impact factor: 3.490
  9 in total
  2 in total

1.  Genetic analysis of Saccharomyces cerevisiae SY 15 relaxed mutant.

Authors:  L I Stateva; P V Venkov
Journal:  Mol Gen Genet       Date:  1984

2.  The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.

Authors:  A A Bayev; O I Georgiev; A A Hadjiolov; M B Kermekchiev; N Nikolaev; K G Skryabin; V M Zakharyev
Journal:  Nucleic Acids Res       Date:  1980-11-11       Impact factor: 16.971
  2 in total

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