Literature DB >> 3902975

A new conjugate for the ELISA quantitation of porcine IgA.

S Bernard, I Lantier.   

Abstract

The preparation of a new soluble immune complex conjugate (anti-IgA-IgA-peroxidase), suitable for use in the ELISA technique is described. This conjugate requires pure IgA and specific anti-IgA, but the enzyme (peroxidase) was easily conjugated to the IgA.

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Year:  1985        PMID: 3902975      PMCID: PMC7130509          DOI: 10.1016/0022-1759(85)90062-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  10 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

3.  Immunoglobulin quantitation in sow serum, colostrum and milk and the serum of young pigs.

Authors:  J Curtis; F J Bourne
Journal:  Biochim Biophys Acta       Date:  1971-04-27

4.  The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.

Authors:  L A Sternberger; P H Hardy; J J Cuculis; H G Meyer
Journal:  J Histochem Cytochem       Date:  1970-05       Impact factor: 2.479

5.  The differential localization of IgA, IgM and IgG in the gut of suckled neonatal piglets.

Authors:  J E Butler; F Klobasa; E Werhahn
Journal:  Vet Immunol Immunopathol       Date:  1981-02       Impact factor: 2.046

6.  Application of the amplified enzyme-linked immunosorbent assay: comparative quantitation of bovine serum IgG1, IgG2, IgA, and IgM antibodies.

Authors:  J E Butler; P L McGivern; L A Cantarero; L Peterson
Journal:  Am J Vet Res       Date:  1980-09       Impact factor: 1.156

7.  Possible factors influencing immunoglobulin A concentration in swine colostrum.

Authors:  T Inoue
Journal:  Am J Vet Res       Date:  1981-03       Impact factor: 1.156

8.  Antibody-dependent cell-mediated antibacterial activity of intestinal lymphocytes with secretory IgA.

Authors:  A Tagliabue; L Nencioni; L Villa; D F Keren; G H Lowell; D Boraschi
Journal:  Nature       Date:  1983 Nov 10-16       Impact factor: 49.962

9.  [Experimental infection of fattening swine with rotavirus: study of local immunity using coproantibodies].

Authors:  S Bernard; J Groom; I Lantier
Journal:  Ann Rech Vet       Date:  1984

10.  A convenient replacement for cyanogen bromide-activated solid phases in immunoradiometric assays.

Authors:  J F Wright; W M Hunter
Journal:  J Immunol Methods       Date:  1982       Impact factor: 2.303

  10 in total
  3 in total

1.  Lactogenic immunity to transmissible gastroenteritis (TGE) of swine induced by the attenuated Nouzilly strain of TGE virus: passive protection of piglets and detection of serum and milk antibody classes by ELISA.

Authors:  S Bernard; J Shirai; I Lantier; E Bottreau; J M Aynaud
Journal:  Vet Immunol Immunopathol       Date:  1990-01       Impact factor: 2.046

2.  Kinetics of the in vitro antibody response to transmissible gastroenteritis (TGE) virus from pig mesenteric lymph node cells, using the ELISASPOT and ELISA tests.

Authors:  P Berthon; S Bernard; H Salmon; R M Binns
Journal:  J Immunol Methods       Date:  1990-08-07       Impact factor: 2.303

3.  Transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains.

Authors:  L T To; S Bernard; E Bottreau
Journal:  Res Virol       Date:  1992 Jul-Aug
  3 in total

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