Literature DB >> 3902766

Hemolytic complement activity assay in microtitration plates.

V R Muzykantov, G P Samokhin, M D Smirnov, S P Domogatsky.   

Abstract

A new rapid technique is developed for the determination of complement activity in a large number of samples. Following serial dilution of complement, hemolysis is performed in the same microtiter plate. After the reaction, the degree of hemolysis in wells of the plate is determined spectrophotometrically by measurement of "absorbance" (light scattering) at 630 nm, without additional procedures. This method can find application in clinical and experimental biochemistry for the analysis of a large (up to thousands) number of samples.

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Year:  1985        PMID： 3902766

Source DB:  PubMed          Journal:  J Appl Biochem        ISSN： 0161-7354


  4 in total

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Journal:  J Vis Exp       Date:  2013-01-02       Impact factor: 1.355

2.  Avidin acylation prevents the complement-dependent lysis of avidin-carrying erythrocytes.

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Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857

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Authors:  P S Unnikrishnan; K Suthindhiran; M A Jayasri
Journal:  Biomed Res Int       Date:  2014-06-24       Impact factor: 3.411

4.  The Effect of Polymeric Nanoparticles on Biocompatibility of Carrier Red Blood Cells.

Authors:  Daniel Pan; Omayra Vargas-Morales; Blaine Zern; Aaron C Anselmo; Vivek Gupta; Michael Zakrewsky; Samir Mitragotri; Vladimir Muzykantov
Journal:  PLoS One       Date:  2016-03-22       Impact factor: 3.240
  4 in total

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