Assignment of the low-field 1H NMR spectrum of Escherichia coli tRNAPhe using nuclear Overhauser effects.

The imino region of the proton NMR spectrum of Escherichia coli tRNAPhe has been largely assigned from the nuclear Overhauser effects between neighboring bases. These have led to the unambiguous assignment of the imino protons of the ribothymidine stem and of most of the dihydrouridine stem of this tRNA and given several other sets of connectivities. These connectivities are discussed in reference to the previously reported temperature studies of the spectrum [Hurd, R. E., & Reid, B. R. (1980) J. Mol. Biol. 142, 1981] and compared with assignments of other tRNAs resulting in tentative assignments of the rest of the spectrum.