Literature DB >> 3901626

[Cloning and the expression of the hemolysin gene of Leptospira pomona pomona in Escherichia coli].

A A Daĭn, M N Rozinov, T A Gol'tsmaĭer, V N Gershanovich, Iu G Chernukha.   

Abstract

The library of Leptospira pomona genes was obtained on phage vector AL 47.1. From this library a recombinant phage carrying the hemolysin gene was selected. The DNA fragment (7.7 kb) of this phage containing the hemolysin gene was subcloned on plasmid pUC19. E. coli clones with hybrid plasmid pDR7 were shown to be hemolytic, but the secretion of hemolysin by E. coli into the culture medium was not observed.

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Year:  1985        PMID: 3901626

Source DB:  PubMed          Journal:  Zh Mikrobiol Epidemiol Immunobiol        ISSN: 0372-9311


  2 in total

1.  Molecular analysis of a sphingomyelinase C gene from Leptospira interrogans serovar hardjo.

Authors:  R P Segers; A van der Drift; A de Nijs; P Corcione; B A van der Zeijst; W Gaastra
Journal:  Infect Immun       Date:  1990-07       Impact factor: 3.441

2.  Presence of putative sphingomyelinase genes among members of the family Leptospiraceae.

Authors:  R P Segers; J A van Gestel; G J van Eys; B A van der Zeijst; W Gaastra
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441

  2 in total

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