| Literature DB >> 3900062 |
D E Brash, W A Franklin, G B Sancar, A Sancar, W A Haseltine.
Abstract
The effect of purified Escherichia coli DNA photolyase on the UV light-induced pyrimidine-pyrimidone (6-4) photoproduct and cyclobutane pyrimidine dimer was investigated in vitro using enzyme purified from cells carrying the cloned phr gene (map position, 15.7 min). Photoproducts were examined both as site-specific lesions in end-labeled DNA and as chromatographically identified products in uniformly labeled DNA. E. coli DNA photolyase removed cyclobutane dimers but had no activity on pyrimidine-pyrimidone (6-4) photoproducts. Photoreactivation can therefore be used to separate the biological effects of these two UV light-induced molecular lesions.Entities:
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Year: 1985 PMID： 3900062
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157