| Literature DB >> 3900049 |
J W Wills, W B Troutman, W S Riggsby.
Abstract
The mitochondrial DNA (mtDNA) of the dimorphic fungus Candida albicans has a molecular size of 41 kilobase pairs as judged by summation of the fragment sizes produced by digestion with restriction endonucleases EcoRI, PvuII, and a combination of both enzymes. Five of the six EcoRI fragments comprising the mitochondrial genome have been cloned into the plasmid vector, pBR322. Restriction mapping revealed a circular map as predicted by previous observations with the electron microscope. The use of nick-translated, purified mtDNA to probe digests of mtDNA from other strains of C. albicans revealed a common restriction pattern. Use of nick-translated, cloned EcoRI fragments to probe digests of mtDNA revealed a large (at least 5 kilobase pairs), inverted duplication as well as a smaller (less than 0.4 kilobase pairs) region of related sequences.Entities:
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Year: 1985 PMID： 3900049 PMCID： PMC214203 DOI： 10.1128/jb.164.1.7-13.1985
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490