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Literature DB >> 3897462

Characterization of a monoclonal antibody to myosin specific for mammalian and human type II muscle fibers.

Abstract

We have characterized a monoclonal antibody (McAb), ALD-19, generated against slow myosin from chicken anterior latissimus dorsi (ALD) muscle for use in studies of human and animal muscle fiber types. This McAb bound selectively to the 200 kDa myosin heavy chain band in immunoblots against chicken, rat and human myosins and showed selective staining of A bands in the myofibrils. The reactivity of ALD-19 with various myosin types was quantitated by radioimmunoassays. Fiber type analysis revealed unexpected specificity of McAb ALD-19 for type II mammalian muscle fibers. This antibody should, therefore be useful for identification and quantification of normal type II fibers in human muscle biopsy specimens.

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Year: 1985 PMID： 3897462 DOI： 10.1016/0022-510x(85)90137-6

Source DB: PubMed Journal: J Neurol Sci ISSN： 0022-510X Impact factor: 3.181

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Cited

15 in total

1. Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.

Authors: P S Stål; R Lindman
Journal: J Anat Date: 2000-08 Impact factor: 2.610

2. Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii.

Authors: Catharina Osterlund; Mona Lindström; Lars-Eric Thornell; Per-Olof Eriksson
Journal: Histochem Cell Biol Date: 2012-07-10 Impact factor: 4.304

10. Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.

Authors: Catharina Österlund; Jing-Xia Liu; Lars-Eric Thornell; Per-Olof Eriksson
Journal: Histochem Cell Biol Date: 2013-01-11 Impact factor: 4.304

Characterization of a monoclonal antibody to myosin specific for mammalian and human type II muscle fibers.

1. Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply.

2. Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii.

3. Expression of an alpha cardiac-like myosin heavy chain in muscle spindle fibres.

4. Monoclonal antibody that detects human type I muscle fibres in routinely fixed wax embedded sections.

5. An immunocytochemical marker for early type I muscle fibers in the developing rat hindlimb.

6. Origin of intrafusal fibers from a subset of primary myotubes in the rat.

7. Familial fetal akinesia deformation sequence with a skeletal muscle maturation defect.

8. Distribution of SERCA isoforms in human intrafusal fibers.

9. Transient expression of a slow-tonic MHC isoform by extrafusal fibers in the developing rat.

10. Intrafusal myosin heavy chain expression of human masseter and biceps muscles at young age shows fundamental similarities but also marked differences.