Sampling and isolation of Cryptococcus neoformans from indoor air with the aid of the Reuter Centrifugal Sampler (RCS) and guizotia abyssinica creatinine agar. A contribution to the mycological-epidemiological control of Cr. neoformans in the fecal matter of caged birds.

In February and March 1984, Cryptococcus neoformans was detected in the manure and ambient air of a volery in the Berlin Zoo in which a Palm Cockatoo (Probosciger aterrimus) was kept. Both times, 5 colony-forming units of Cr. neoformans could be isolated on the differential medium, Guizotia abyssinica creatinine agar, from 40 1 of air aspirated by the Reuter Centrifugal Sampler (RCS). The absence of a concurrent growth of moulds was found to be of special importance for the optimal isolation of Cr. neoformans on the above mentioned agar for purposes of epidemiological research into airborne dissemination of the fungus. The advantages and disadvantages of 0.1% biphenyl to inhibit concurring growth of moulds are discussed. The control of habitats and foci of Cr. neoformans in zoos and similar establishments is considered a necessity, to prevent inhalatory exposure of susceptible, e.g. immuno-compromised persons.