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Literature DB >> 3894263

Comparative reduction/oxidation studies with single chain des-(B30) insulin and porcine proinsulin.

Abstract

The single chain des-(B30) insulin molecule (SCI) has been reduced and reoxidized together with porcine proinsulin (PPI). Yields of correctly folded and reoxidized SCI and PPI were analyzed by HPLC. The concentrations of both proteins were 10(-3) M during reduction and 10(-5) M during oxidation. The pH during reoxidation was varied from 8.6 to 9.2 and the temperature from 4 to 37 degrees. Under all conditions tested, the recovery of SCI was substantially higher than that of PPI. The recoveries peaked after 24-72 h. It is suggested that the "miniproinsulin" SCI folds correctly up more efficiently than porcine proinsulin, resulting in higher yields of reoxidized SCI.

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Year: 1985 PMID： 3894263 DOI： 10.1111/j.1399-3011.1985.tb02197.x

Source DB: PubMed Journal: Int J Pept Protein Res ISSN： 0367-8377

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Cited

11 in total

1. Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications.

Authors: Qing-xin Hua; Satoe H Nakagawa; Wenhua Jia; Kun Huang; Nelson B Phillips; Shi-quan Hu; Michael A Weiss
Journal: J Biol Chem Date: 2008-03-10 Impact factor: 5.157

2. Fully convergent chemical synthesis of ester insulin: determination of the high resolution X-ray structure by racemic protein crystallography.

Authors: Michal Avital-Shmilovici; Kalyaneswar Mandal; Zachary P Gates; Nelson B Phillips; Michael A Weiss; Stephen B H Kent
Journal: J Am Chem Soc Date: 2013-02-08 Impact factor: 15.419

3. Backbone cyclization of analgesic conotoxin GeXIVA facilitates direct folding of the ribbon isomer.

Authors: Xiaosa Wu; Yen-Hua Huang; Quentin Kaas; Peta J Harvey; Conan K Wang; Han-Shen Tae; David J Adams; David J Craik
Journal: J Biol Chem Date: 2017-08-28 Impact factor: 5.157

4. Chiral mutagenesis of insulin. Foldability and function are inversely regulated by a stereospecific switch in the B chain.

Authors: Satoe H Nakagawa; Ming Zhao; Qing-xin Hua; Shi-Quan Hu; Zhu-li Wan; Wenhua Jia; Michael A Weiss
Journal: Biochemistry Date: 2005-04-05 Impact factor: 3.162

5. Intra-A chain disulphide bond forms first during insulin precursor folding.

Authors: Y Yuan; Z H Wang; J G Tang
Journal: Biochem J Date: 1999-10-01 Impact factor: 3.857

6. Contribution of residue B5 to the folding and function of insulin and IGF-I: constraints and fine-tuning in the evolution of a protein family.

Authors: Youhei Sohma; Qing-xin Hua; Ming Liu; Nelson B Phillips; Shi-Quan Hu; Jonathan Whittaker; Linda J Whittaker; Aubree Ng; Charles T Roberts; Peter Arvan; Stephen B H Kent; Michael A Weiss
Journal: J Biol Chem Date: 2009-12-03 Impact factor: 5.157

Comparative reduction/oxidation studies with single chain des-(B30) insulin and porcine proinsulin.

1. Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications.

2. Fully convergent chemical synthesis of ester insulin: determination of the high resolution X-ray structure by racemic protein crystallography.

3. Backbone cyclization of analgesic conotoxin GeXIVA facilitates direct folding of the ribbon isomer.

4. Chiral mutagenesis of insulin. Foldability and function are inversely regulated by a stereospecific switch in the B chain.

5. Intra-A chain disulphide bond forms first during insulin precursor folding.

6. Contribution of residue B5 to the folding and function of insulin and IGF-I: constraints and fine-tuning in the evolution of a protein family.

Review 7. Diabetes mellitus due to the toxic misfolding of proinsulin variants.

8. A Bystander Mechanism Explains the Specific Phenotype of a Broadly Expressed Misfolded Protein.

Review 9. Structural Lessons From the Mutant Proinsulin Syndrome.

Review 10. History and diagnostic significance of C-peptide.