Literature DB >> 3893198

Analysis of human alcohol- and aldehyde-metabolizing isozymes by electrophoresis and isoelectric focusing.

J A Duley, O Harris, R S Holmes.   

Abstract

Isoelectric focusing and electrophoresis were used to identify the various isozymes of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH), aldehyde oxidase (AOX), and xanthine oxidase (XOX). ADH types I, II, and III were located primarily in the cytosol fraction of liver, but some activity was found also in the small granule fraction. The ALDH-I and -IV isozymes were found in the large granule fraction, while ALDH-II and -III were present in the cytosol and ALDH-V in the small granule fraction. AOX and XOX each appeared as a single cytosolic form with some small granule activity. The tissue distribution of these isozymes is presented and the physiological role of each enzyme is discussed.

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Year:  1985        PMID： 3893198     DOI： 10.1111/j.1530-0277.1985.tb05747.x

Source DB:  PubMed          Journal:  Alcohol Clin Exp Res        ISSN： 0145-6008            Impact factor:   3.455


  12 in total

1.  Absolute quantification of aldehyde oxidase protein in human liver using liquid chromatography-tandem mass spectrometry.

Authors:  John T Barr; Jeffrey P Jones; Carolyn A Joswig-Jones; Dan A Rock
Journal:  Mol Pharm       Date:  2013-09-19       Impact factor: 4.939

2.  Biochemical genetics of alcohol dehydrogenase isozymes in the gray short-tailed opossum (Monodelphis domestica).

Authors:  R S Holmes; R A van Oorschot; J L VandeBerg
Journal:  Biochem Genet       Date:  1992-06       Impact factor: 1.890

3.  Kinetic evidence for human liver and stomach aldehyde dehydrogenase-3 representing an unique class of isozymes.

Authors:  S J Yin; C S Liao; S L Wang; Y J Chen; C W Wu
Journal:  Biochem Genet       Date:  1989-06       Impact factor: 1.890

4.  The human aldehyde dehydrogenase 3 gene (ALDH3): identification of a new exon and diverse mRNA isoforms, and functional analysis of the promoter.

Authors:  L C Hsu; W C Chang; C Chang; N Tsukamoto; A Yoshida
Journal:  Gene Expr       Date:  1996

5.  Enhancement of aldehyde dehydrogenase activity in human and rat hepatocyte cultures by 3-methylcholanthrene.

Authors:  M Marselos; S C Strom; G Michalopoulos
Journal:  Cell Biol Toxicol       Date:  1986-06       Impact factor: 6.691

6.  Subcellular localization of aldehyde dehydrogenase isozymes in human liver.

Authors:  S Takase; M Tsutsumi; A Takada
Journal:  Gastroenterol Jpn       Date:  1989-02

7.  Research on alcohol metabolism among Asians and its implications for understanding causes of alcoholism.

Authors:  R F Suddendorf
Journal:  Public Health Rep       Date:  1989 Nov-Dec       Impact factor: 2.792

8.  Aldehyde dehydrogenase (ALDH) isozymes in the gray short-tailed opossum (Monodelphis domestica): tissue and subcellular distribution and biochemical genetics of ALDH3.

Authors:  R S Holmes; R A van Oorschot; J L VandeBerg
Journal:  Biochem Genet       Date:  1991-04       Impact factor: 1.890

9.  Genetics of ocular NAD+-dependent alcohol dehydrogenase and aldehyde dehydrogenase in the mouse: evidence for genetic identity with stomach isozymes and localization of Ahd-4 on chromosome 11 near trembler.

Authors:  R S Holmes; R A Popp; J L VandeBerg
Journal:  Biochem Genet       Date:  1988-04       Impact factor: 1.890

10.  The mammalian aldehyde oxidase gene family.

Authors:  Enrico Garattini; Maddalena Fratelli; Mineko Terao
Journal:  Hum Genomics       Date:  2009-12       Impact factor: 4.639
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