Literature DB >> 3891860

MicroELISA method for measurement of human serum thymosin alpha 1.

F E Weller, M G Mutchnick, D F Keren, A L Goldstein, P H Naylor.   

Abstract

A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring serum levels of thymosin alpha 1 and highly reproducible. The serum levels measured with the microELISA are comparable to serum levels of thymosin alpha 1 determined by the previously described radioimmunoassay for thymosin alpha 1.

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Year:  1985        PMID: 3891860     DOI: 10.1016/0022-1759(85)90163-2

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  1 in total

1.  Cellular levels of thymosin immunoreactive peptides are linked to proliferative events: evidence for a nuclear site of action.

Authors:  C N Conteas; M G Mutchnick; K C Palmer; F E Weller; G D Luk; P H Naylor; M R Erdos; A L Goldstein; C Panneerselvam; B L Horecker
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

  1 in total

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