Literature DB >> 3890354

Enzymatic radioimmunoassay for detecting Leptospira interrogans serovar pomona in the urine of experimentally-infected pigs.

R J Chappel, B Adler, S A Ballard, S Faine, R T Jones, B D Millar, J A Swainger.   

Abstract

An enzymatic radioimmunoassay (ERIA) has been developed for detecting Leptospira interrogans serovar pomona in porcine urine. Four grower pigs were experimentally infected with serovar pomona. A total of 39 urine samples was collected, and ERIA was compared with dark ground microscopy (DGM) and culture for demonstrating leptospiruria. Of 20 samples positive by at least one technique, leptospires were detected by ERIA in 14, by culture in 16 and by DGM in 13. ERIA, unlike the other 2 methods, was suitable for use with urine which had been stored frozen for several months.

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Year:  1985        PMID: 3890354     DOI: 10.1016/0378-1135(85)90053-7

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN: 0378-1135            Impact factor:   3.293


  4 in total

Review 1.  Leptospirosis.

Authors:  P N Levett
Journal:  Clin Microbiol Rev       Date:  2001-04       Impact factor: 26.132

2.  Detection and characterization of leptospiral antigens using a biotin/avidin double-antibody sandwich enzyme-linked immunosorbent assay and immunoblot.

Authors:  M J Champagne; R Higgins; J M Fairbrother; D Dubreuil
Journal:  Can J Vet Res       Date:  1991-07       Impact factor: 1.310

Review 3.  The role of nuclear technologies in the diagnosis and control of livestock diseases--a review.

Authors:  Gerrit J Viljoen; Antony G Luckins
Journal:  Trop Anim Health Prod       Date:  2012-10       Impact factor: 1.559

Review 4.  Moving past serology: Diagnostic options without serum.

Authors:  Michael P Reichel; Sasha R Lanyon; Fraser I Hill
Journal:  Vet J       Date:  2016-04-22       Impact factor: 2.688

  4 in total

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