Literature DB >> 3889068

Localisation of Ca and HMFG2 antigens in breast tissue by immunoperoxidase, immunofluorescence, and immunoelectron microscopy.

U Beckford, C Barbatis, J E Beesley, J C Linsell, S M Chantler.   

Abstract

The reactivities of Ca1 and HMFG2 monoclonal antibodies were compared on paraffin wax embedded breast tissues using indirect immunoperoxidase. The expression of Ca antigen, like HMFG2, is not exclusive to malignancy: Ca was present in 41/53 (77%) and HMFG2 in 42/53 (79.2%) non-malignant conditions and both were present in 33/35 (94%) carcinomas. Similar results were obtained when cryostat sections were used. Both antigens showed striking similarities in their topographical distributions, although quantitative differences were seen. Their cellular and sub-cellular localisations were investigated by double labelling immunofluorescence and immunogold electron microscopy, which showed that the expression of Ca and HMFG2 antigens was closely associated on cell membranes but that the epitopes were distinct.

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Year:  1985        PMID： 3889068      PMCID： PMC499200          DOI： 10.1136/jcp.38.5.512

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  18 in total

1.  Immunocytochemical labelling of Bacteroides nodosus pili using an immunogold technique.

Authors:  J E Beesley; S E Day; M P Betts; C M Thorley
Journal:  J Gen Microbiol       Date:  1984-06

2.  Cal antibody applied to cytological diagnosis of fine needle aspirates from breast lumps.

Authors:  A K Ghosh; A I Spriggs; M M Boddington; H B Ross
Journal:  Lancet       Date:  1983-07-30       Impact factor: 79.321

3.  Complexity of expression of antigenic determinants, recognized by monoclonal antibodies HMFG-1 and HMFG-2, in normal and malignant human mammary epithelial cells.

Authors:  J Burchell; H Durbin; J Taylor-Papadimitriou
Journal:  J Immunol       Date:  1983-07       Impact factor: 5.422

4.  Monoclonal antibodies to the human mammary gland. I. Distribution of determinants in non-neoplastic mammary and extra mammary tissues.

Authors:  C S Foster; P A Edwards; E A Dinsdale; A M Neville
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1982

5.  A simple method of reducing the fading of immunofluorescence during microscopy.

Authors:  G D Johnson; G M Nogueira Araujo
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

6.  Monoclonal antibodies to the human mammary gland. II. Distribution of determinants in breast carcinomas.

Authors:  C S Foster; E A Dinsdale; P A Edwards; A M Neville
Journal:  Virchows Arch A Pathol Anat Histol       Date:  1982

7.  A new marker for human cancer cells. 1 The Ca antigen and the Ca1 antibody.

Authors:  F Ashall; M E Bramwell; H Harris
Journal:  Lancet       Date:  1982-07-03       Impact factor: 79.321

8.  A new marker for human cancer cells, 2 immunohistochemical detection of the Ca antigen in human tissues with the Ca1 antibody.

Authors:  J O McGee; J C Woods; F Ashall; M E Bramwell; H Harris
Journal:  Lancet       Date:  1982-07-03       Impact factor: 79.321

9.  Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue.

Authors:  M Naiem; J Gerdes; Z Abdulaziz; H Stein; D Y Mason
Journal:  J Clin Pathol       Date:  1983-02       Impact factor: 3.411

10.  Structure and function of the Ca antigen.

Authors:  M E Bramwell; V P Bhavanandan; G Wiseman; H Harris
Journal:  Br J Cancer       Date:  1983-08       Impact factor: 7.640
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  2 in total

1.  Can monoclonal antibody staining by 323/A3 and Ca1 of benign breast biopsies predict the development of breast cancer?

Authors:  S P Courtney; S Williams; R E Mansel
Journal:  J Cancer Res Clin Oncol       Date:  1993       Impact factor: 4.553

2.  Detection of Ca antigen in sera from normal individuals and patients with benign and malignant breast disease.

Authors:  A B Goodall; C J Evans; D Trivedi; R C Coombes; S M Chantler
Journal:  Br J Cancer       Date:  1985-08       Impact factor: 7.640
  2 in total

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