Literature DB >> 3886381

In vitro transcription initiation from three different Escherichia coli promoters. Effect of supercoiling.

R Ehrlich, A Larousse, M A Jacquet, M Marin, C Reiss.   

Abstract

Transcription initiation from beta-lactamase, tetracycline resistance and RNA 1 promoters, present in plasmid pAT153, were studied employing the abortive initiation technique. Assays appear to be promoter-specific with supercoiled and linear templates. Supercoiling enhances the isomerization rate constant of the open RNA-polymerase--promoter complex formation. Results agree with the in vivo behaviour of the corresponding promoters, and allow us to propose a hypothesis about the effect of supercoiling on transcription initiation.

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Year:  1985        PMID： 3886381     DOI： 10.1111/j.1432-1033.1985.tb08838.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  12 in total

1.  Effect of the sequence-dependent structure of the 17 bp AT spacer on the strength of consensuslike E.coli promoters in vivo.

Authors:  T Lozinski; W T Markiewicz; T K Wyrzykiewicz; K L Wierzchowski
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971

2.  Mitochondrial DNA structure and expression in specialized subtypes of mammalian striated muscle.

Authors:  B H Annex; R S Williams
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

3.  Binding of BAL 31 RNA polymerase to PM2 DNA as determined by electron microscopy and protection against restriction endonuclease cleavage.

Authors:  P Bull; M Susaeta; B González; A Yudelevich
Journal:  J Virol       Date:  1988-10       Impact factor: 5.103

4.  Dynamic and structural characterisation of multiple steps during complex formation between E. coli RNA polymerase and the tetR promoter from pSC101.

Authors:  G Duval-Valentin; R Ehrlich
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

5.  Interaction between E. coli RNA polymerase and the tetR promoter from pSC101: homologies and differences with other E. coli promoter systems from close contact point studies.

Authors:  G Duval-Valentin; R Ehrlich
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

6.  Far upstream sequences of the bla promoter from TN3 are involved in complexation with E. coli RNA-polymerase.

Authors:  G Duval-Valentin; R Ehrlich
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

7.  A second RNA-polymerase can bind specifically to the bla promoter of Tn3, repressing transcription initiation.

Authors:  G Duval-Valentin; B Schmitt; R Ehrlich
Journal:  Nucleic Acids Res       Date:  1988-06-24       Impact factor: 16.971

8.  Kinetic study in vitro of Escherichia coli promoter closure during transcription initiation.

Authors:  B Schmitt; C Reiss
Journal:  Biochem J       Date:  1995-02-15       Impact factor: 3.857

9.  Kinetics of the specific binding of a second RNA polymerase to the standard bacterial-transposon-Tn3 bla promoter complex.

Authors:  B Schmitt; C Reiss
Journal:  Biochem J       Date:  1991-07-15       Impact factor: 3.857

10.  Specific inhibition of transcription by triple helix-forming oligonucleotides.

Authors:  G Duval-Valentin; N T Thuong; C Hélène
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205
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