Literature DB >> 3885942

Expression of the cloned subunits of bacterial luciferase from separate replicons.

S C Gupta, D O'Brien, J W Hastings.   

Abstract

The lux A and lux B genes of Vibrio harveyi, encoding the alpha and beta subunits of bacterial luciferase, were cloned individually into Escherichia coli in two different compatible plasmids. Active luciferase was formed in an amount equal to that produced in cells carrying a plasmid with the cloned genes on a single fragment.

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Year:  1985        PMID: 3885942     DOI: 10.1016/s0006-291x(85)80044-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN: 0006-291X            Impact factor:   3.575


  1 in total

1.  Bacterial luciferase alpha beta fusion protein is fully active as a monomer and highly sensitive in vivo to elevated temperature.

Authors:  A Escher; D J O'Kane; J Lee; A A Szalay
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

  1 in total

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