Literature DB >> 3885854

Method for improving the detection of viruses in fecal samples.

R D Pontefract, G Bergeron.   

Abstract

A method is described that improved the detection of viruses in fecal samples by electron microscopy. The virus particles were concentrated, and much of the background debris was removed by adsorption of viruses on meat protein added to the fecal sample at a low pH and a low salt concentration. Viruses were eluted by raising the pH and the salt concentration. Further concentration was achieved by acid precipitation and vacuum dialysis.

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Year:  1985        PMID: 3885854      PMCID: PMC238427          DOI: 10.1128/aem.49.2.456-458.1985

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  3 in total

1.  Improved methods for detecting enteric viruses in oysters.

Authors:  M D Sobsey; R J Carrick; H R Jensen
Journal:  Appl Environ Microbiol       Date:  1978-07       Impact factor: 4.792

2.  A simple technique for the removal of formvar from carbon-formvar coated grids.

Authors:  R D Pontefract; G Bergeron
Journal:  Stain Technol       Date:  1981-01

3.  Agar diffusion method for negative staining of microbial suspensions in salt solutions.

Authors:  N Anderson; F W Doane
Journal:  Appl Microbiol       Date:  1972-09
  3 in total

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