| Literature DB >> 3885785 |
Abstract
After beta-lactamase had been denatured by boiling in the presence of sodium dodecyl sulfate (SDS) and then electrophoresed in SDS-polyacrylamide gels, activity could be restored and could be detected in situ as specific molecular species. Renaturation was simple and facilitated by the presence of a carrier protein. The assay was sensitive, detecting 0.8 ng beta-lactamase activity in the gel.Entities:
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Year: 1985 PMID： 3885785 DOI： 10.1016/0003-2697(85)90105-8
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365