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Literature DB >> 3885067

A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane.

Abstract

An efficient and reliable method for preparing chromaffin granules and membranes for topographical analysis has been designed to meet the following criteria. Only labeling of truly intact organelles is analyzed for cytoplasmic surface probes. No resealing of open membranes occurs under specified reaction conditions. Saturation of available sites must be demonstrated. Problems in meeting these conditions are discussed, in particular the interference of granule lysate with labeling reactions.

Entities: Chemical

Mesh：

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Year: 1985 PMID： 3885067 DOI： 10.1007/bf00964769

Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996

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References

13 in total

1. A fluorimetric micromethod for differential estimation of adrenaline and noradrenaline.

Authors: U S VON EULER; I FLODING
Journal: Acta Physiol Scand Suppl Date: 1955

2. Adrenal chromaffin granules: isolation and disassembly.

Authors: S F Bartlett; A D Smith
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

3. The effect of salts on catecholamine fluxes and adenosine triphosphatase activity in storage vesicles from the adrenal medulla.

Authors: G Taugner
Journal: Biochem J Date: 1971-06 Impact factor: 3.857

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. The arrangement of proteins in the human erythrocyte membrane.

Authors: D R Phillips; M Morrison
Journal: Biochem Biophys Res Commun Date: 1970-07-27 Impact factor: 3.575

6. 3,4-dihydroxyphenylethylamine beta-hydroxylase. Physical properties, copper content, and role of copper in the catalytic acttivity.

Authors: S Friedman; S Kaufman
Journal: J Biol Chem Date: 1965-12 Impact factor: 5.157

A reliable method for assessing topographical arrangement of proteins in the chromaffin granule membrane.

1. A fluorimetric micromethod for differential estimation of adrenaline and noradrenaline.

2. Adrenal chromaffin granules: isolation and disassembly.

3. The effect of salts on catecholamine fluxes and adenosine triphosphatase activity in storage vesicles from the adrenal medulla.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. The arrangement of proteins in the human erythrocyte membrane.

6. 3,4-dihydroxyphenylethylamine beta-hydroxylase. Physical properties, copper content, and role of copper in the catalytic acttivity.

7. The molecular organization of adrenal chromaffin granules.

8. Transmembrane nature of chromaffin granule dopamine beta-monooxygenase.

9. Organisation of the proteins of the chromaffin granule membrane.

10. Transport of catecholamines by resealed chromaffin-grnaule "ghosts".