Literature DB >> 3884644

Simple detection of antibodies to different viruses using rheumatoid factor and enzyme-labelled antigen (ELA).

M Sachers, P Emmerich, H Mohr, H Schmitz.   

Abstract

A new one-step method was developed to detect IgG antibodies to different virus antigens. Human serum specimens were mixed with different enzyme-labelled antigen (ELA) preparations in microtitre plates coated with rheumatoid factor (RF). During the incubation in the wells of microtitre plates immune complexes form which are specifically bound to the RF on the solid phase. The test detects antibody to cytomegalovirus (CMV) and West-Nile virus with a high sensitivity and specificity.

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Year:  1985        PMID: 3884644     DOI: 10.1016/0166-0934(85)90095-3

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  5 in total

1.  Use of monoclonal antibody for the detection of Lassa virus antibody and antigen in patients with Lassa fever.

Authors:  H Schmitz; H R Wolf
Journal:  Med Microbiol Immunol       Date:  1986       Impact factor: 3.402

2.  Specific detection of antibodies to different flaviviruses using a new immune complex ELISA.

Authors:  Herbert Schmitz; Martin Gabriel; Petra Emmerich
Journal:  Med Microbiol Immunol       Date:  2011-05-01       Impact factor: 3.402

3.  Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G.

Authors:  Martin Gabriel; Donatus I Adomeh; Jacqueline Ehimuan; Jennifer Oyakhilome; Emmanuel O Omomoh; Yemisi Ighodalo; Thomas Olokor; Kofi Bonney; Meike Pahlmann; Petra Emmerich; Michaela Lelke; Linda Brunotte; Stephan Ölschläger; Corinna Thomé-Bolduan; Beate Becker-Ziaja; Carola Busch; Ikponmwosa Odia; Ephraim Ogbaini-Emovon; Peter O Okokhere; Sylvanus A Okogbenin; George O Akpede; Herbert Schmitz; Danny A Asogun; Stephan Günther
Journal:  PLoS Negl Trop Dis       Date:  2018-03-29

4.  Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)-Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests.

Authors:  Petra Emmerich; Angela Mika; Ronald von Possel; Anne Rackow; Yang Liu; Herbert Schmitz; Stephan Günther; Kurtesh Sherifi; Barie Halili; Xhevat Jakupi; Lindita Berisha; Salih Ahmeti; Christina Deschermeier
Journal:  PLoS Negl Trop Dis       Date:  2018-03-26

5.  Reverse ELISA for the detection of anti West Nile virus IgG antibodies in humans.

Authors:  D Ludolfs; M Niedrig; J T Paweska; H Schmitz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-07       Impact factor: 5.103

  5 in total

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