Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Escherichia coli mutants possessing an Li+-resistant melibiose carrier.

Literature DB >> 3884586

Escherichia coli mutants possessing an Li+-resistant melibiose carrier.

S Shiota, Y Yamane, M Futai, T Tsuchiya.

Abstract

Escherichia coli K-12 strains in the absence of the lactose carrier grew on the disaccharide melibiose as the sole source of carbon. The presence of 0.1 mM Li+ in the medium strongly inhibited growth of such cells, and Li+-resistant mutants appeared after several days of incubation. These mutants showed altered cation coupling to melibiose transport via the melibiose carrier. Cotransport between H+ and melibiose was lost in the mutants, although Na+-melibiose cotransport was retained. We observed no Li+-melibiose cotransport. Therefore, these mutants represent a new type of cation-coupling mutants of the melibiose carrier.

Entities: Chemical Species

Mesh：

Substances：

Year: 1985 PMID： 3884586 PMCID： PMC218961 DOI： 10.1128/jb.162.1.106-109.1985

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

16 in total

1. Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli.

Authors: T Tsuchiya; J Raven; T H Wilson
Journal: Biochem Biophys Res Commun Date: 1977-05-09 Impact factor: 3.575

9. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol.

Authors: S Tanaka; S A Lerner; E C Lin
Journal: J Bacteriol Date: 1967-02 Impact factor: 3.490

10. Cation-sugar cotransport in the melibiose transport system of Escherichia coli.

Authors: T Tsuchiya; T H Wilson
Journal: Membr Biochem Date: 1978

5 in total

1. Arg-52 in the melibiose carrier of Escherichia coli is important for cation-coupled sugar transport and participates in an intrahelical salt bridge.

Authors: P J Franco; T H Wilson
Journal: J Bacteriol Date: 1999-10 Impact factor: 3.490

2. Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2.

Authors: K Mizushima; S Awakihara; M Kuroda; T Ishikawa; M Tsuda; T Tsuchiya
Journal: Mol Gen Genet Date: 1992-07

3. Asp-51 and Asp-120 are important for the transport function of the Escherichia coli melibiose carrier.

Authors: D M Wilson; T H Wilson
Journal: J Bacteriol Date: 1992-05 Impact factor: 3.490

4. Dissecting the molecular mechanism of ion-solute cotransport: substrate specificity mutations in the putP gene affect the kinetics of proline transport.

Authors: R S Myers; D Townsend; S Maloy
Journal: J Membr Biol Date: 1991-05 Impact factor: 1.843

5. Proline transport in Salmonella typhimurium: putP permease mutants with altered substrate specificity.

Authors: D K Dila; S R Maloy
Journal: J Bacteriol Date: 1986-11 Impact factor: 3.490

5 in total

Escherichia coli mutants possessing an Li+-resistant melibiose carrier.

1. Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli.

2. Protein measurement with the Folin phenol reagent.

3. Sodium-proton antiport in isolated membrane vesicles of Escherichia coli.

4. Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.

5. Galactoside transport dissociated from proton movement in mutants of Escherichia coli.

6. A mutant of Escherichia coli K 12 energy-uncoupled for lactose transport.

7. Inhibitory effect of Li+ on cell growth and pyruvate kinase activity of Escherichia coli.

8. A sodium-dependent sugar co-transport system in bacteria.