| Literature DB >> 3882458 |
V A Efimov, O V Mirskikh, O G Chakhmakhcheva.
Abstract
A new modification of the oligonucleotide-mediated mutagenesis technique has been developed. The proposed methodology has been used to produce specific base changes in the double-stranded plasmid DNA. For this purpose, special cloning vectors have been constructed using the synthetic oligodeoxyribonucleotides. The developed method allows the production of mutant DNA from those of the wild-type with a yield of 10-20%.Entities:
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Year: 1985 PMID： 3882458 DOI： 10.1016/0014-5793(85)80302-1
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124