Literature DB >> 388187

Improved method for the specific immunoprecipitation of albumin.

I D Algranati.   

Abstract

The immunoprecipitates of many antigens are frequently contaminated by coprecipitation of unrelated substances. A method to overcome this type of contamination in the immunoprecipitation of albumin is described. The insoluble albumin-antibody complexes are solubilized by a brief treatment at high temperature in the presence of sodium dodecyl sulfate, and after dilution the mixture is submitted to a second immunoprecipitation.

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Year:  1979        PMID： 388187     DOI： 10.1007/bf00218352

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  8 in total

1.  Comparison of the effects of various detergents on antigen-antibody interaction.

Authors:  M J. Crumpton; R M.E. Parkhouse
Journal:  FEBS Lett       Date:  1972-05-01       Impact factor: 4.124

2.  Interaction of protein antigens and antibodies. I. Inhibition studies with the egg albumin-antiegg albumin system.

Authors:  W J KLEINSCHMIDT; P D BOYER
Journal:  J Immunol       Date:  1952-09       Impact factor: 5.422

3.  Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids.

Authors:  J A Schneider; M C Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  1971-01       Impact factor: 11.205

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

5.  Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Localization in polysomes, induction by estrogen, and effect of actinomycin D.

Authors:  R E Rhoads; G S McKnight; R T Schimke
Journal:  J Biol Chem       Date:  1973-03-25       Impact factor: 5.157

6.  Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system.

Authors:  J M Taylor; R T Schimke
Journal:  J Biol Chem       Date:  1973-11-25       Impact factor: 5.157

7.  Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture.

Authors:  R D Palmiter; T Oka; R T Schimke
Journal:  J Biol Chem       Date:  1971-02-10       Impact factor: 5.157

8.  Selective release of content from microsomal vesicles without membrane disassembly. II. Electrophoretic and immunological characterization of microsomal subfractions.

Authors:  G Kreibich; D D Sabatini
Journal:  J Cell Biol       Date:  1974-06       Impact factor: 10.539
  8 in total

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