| Literature DB >> 3881397 |
P R MacLachlan, K E Sanderson.
Abstract
Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequencies 8- to 630-fold higher than with smooth strains or other rough mutants.Entities:
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Year: 1985 PMID: 3881397 PMCID: PMC214893 DOI: 10.1128/jb.161.1.442-445.1985
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490