Literature DB >> 3881130

Site specificities of three transfer RNA methyltransferases from yeast.

T Sindhuphak, U Hellman, I Svensson.   

Abstract

The site specificities of two distinct tRNA(m1G)methyltransferases and one tRNA(m2G)methyltransferase from yeast have been investigated by heterologous methylation and analysis of purified Escherichia coli tRNAs. The two tRNA(m1G)methyltransferases were found to be specific for sites 9 and 37, respectively. The tRNA(m2G)methyltransferase was specific for site 10. Two of the enzymes were purified by affinity chromatography on tRNA-Sepharose.

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Year:  1985        PMID： 3881130     DOI： 10.1016/0167-4781(85)90030-2

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  4 in total

1.  Identity elements for N2-dimethylation of guanosine-26 in yeast tRNAs.

Authors:  J Edqvist; H Grosjean; K B Stråby
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

2.  Pleiotrophic effects of point mutations in yeast tRNA(Asp) on the base modification pattern.

Authors:  J Edqvist; K B Stråby; H Grosjean
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

3.  The tRNA(guanine-26,N2-N2) methyltransferase (Trm1) from the hyperthermophilic archaeon Pyrococcus furiosus: cloning, sequencing of the gene and its expression in Escherichia coli.

Authors:  F Constantinesco; N Benachenhou; Y Motorin; H Grosjean
Journal:  Nucleic Acids Res       Date:  1998-08-15       Impact factor: 16.971

4.  Enzymatic conversion of guanosine 3' adjacent to the anticodon of yeast tRNAPhe to N1-methylguanosine and the wye nucleoside: dependence on the anticodon sequence.

Authors:  L Droogmans; H Grosjean
Journal:  EMBO J       Date:  1987-02       Impact factor: 11.598
  4 in total

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