Literature DB >> 3879524

Correction factors for the diphenylamine test for deoxyribonucleic acid in yeasts.

R E Subden, A Krizus.   

Abstract

The diphenylamine assay for DNA content of yeast is unsatisfactory due to the presence of a yeast component which inhibits the colour reaction. The inhibitor is found in a wide range of yeast species and has not been identified. The relationships between the degree of inhibition and cell concentration, temperature and time of hydrolysis and extraction have been described. A formula for correction of the inhibition has been derived.

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Year:  1985        PMID： 3879524

Source DB:  PubMed          Journal:  Microbios        ISSN： 0026-2633


  4 in total

1.  Rapid extraction of genomic DNA from medically important yeasts and filamentous fungi by high-speed cell disruption.

Authors:  F M Müller; K E Werner; M Kasai; A Francesconi; S J Chanock; T J Walsh
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

2.  Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication.

Authors:  Y Kamimura; H Masumoto; A Sugino; H Araki
Journal:  Mol Cell Biol       Date:  1998-10       Impact factor: 4.272

3.  Neuroblastic differentiation potential of the human retinoblastoma cell lines Y-79 and WERI-Rb1 maintained in an organ culture system. An immunohistochemical, electron microscopic, and biochemical study.

Authors:  M M Herman; E Perentes; C D Katsetos; F Darcel; A Frankfurter; V P Collins; L A Donoso; L F Eng; P J Marangos; A F Wiechmann
Journal:  Am J Pathol       Date:  1989-01       Impact factor: 4.307

4.  The RFC2 gene, encoding the third-largest subunit of the replication factor C complex, is required for an S-phase checkpoint in Saccharomyces cerevisiae.

Authors:  V N Noskov; H Araki; A Sugino
Journal:  Mol Cell Biol       Date:  1998-08       Impact factor: 4.272
  4 in total

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