Literature DB >> 387879

Cryosectioning of undecalcified tissues for immunofluorescence.

N V Rijntjes, L B Van de Putte, M Van der Pol, P J Guelen.   

Abstract

The present report describes a procedure for preparing 4--6 micrometers cryostat sections of undecalcified fresh frozen tissue which contain hard tissue, for immunofluorescence. The apparatus used is a cryomicrotome originally designed for cutting sections for whole body autoradiography. To obtain cryostat sections suitable for tissue immunofluorescence the standard procedure was modified with respect to the hardness and edges of the microtome knife, the temperature of the cryostat and the carboxymethyl cellulose concentration of the embedding material.

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Year:  1979        PMID： 387879     DOI： 10.1016/0022-1759(79)90100-5

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  13 in total

1.  A novel technique for immunohistoperoxidase staining of unfixed whole joints of small animals.

Authors:  S C Meacock; D R Brandon; C P Brown; B P Swann
Journal:  Histochem J       Date:  1992-02

2.  Mast cells in early stages of antigen-induced arthritis in rat knee joints.

Authors:  A M Tiggelman; C J Van Noorden
Journal:  Int J Exp Pathol       Date:  1990-08       Impact factor: 1.925

3.  Flare-up of antigen-induced arthritis in mice after challenge with intravenous antigen: effects of pre-treatment with cobra venom factor and anti-lymphocyte serum.

Authors:  J W Lens; W B van den Berg; L B van de Putte; J H Berden; S P Lems
Journal:  Clin Exp Immunol       Date:  1984-09       Impact factor: 4.330

4.  Distribution of different fibre types in human skeletal muscles. I. Method for the preparation and analysis of cross-sections of whole tibialis anterior.

Authors:  K B Henriksson-Larsén; J Lexell; M Sjöström
Journal:  Histochem J       Date:  1983-02

5.  Anti-inflammatory effects of systemic anti-tumour necrosis factor alpha treatment in human/murine SCID arthritis.

Authors:  H Schädlich; J Ermann; M Biskop; W Falk; F Sperling; A Jüngel; J Lehmann; F Emmrich; U Sack
Journal:  Ann Rheum Dis       Date:  1999-07       Impact factor: 19.103

6.  Histochemical analysis of insulin-like growth factor-1 binding sites in mouse normal and experimentally induced arthritic articular cartilage.

Authors:  P J Verschure; J Van Marle; L A Joosten; W B Van Den Berg
Journal:  Histochem J       Date:  1996-01

7.  Antigen handling in antigen-induced arthritis in mice: an autoradiographic and immunofluorescence study using whole joint sections.

Authors:  W B van den Berg; H J van Beusekom; L B van de Putte; W A Zwarts; M van der Sluis
Journal:  Am J Pathol       Date:  1982-07       Impact factor: 4.307

8.  Cationization of catalase, peroxidase, and superoxide dismutase. Effect of improved intraarticular retention on experimental arthritis in mice.

Authors:  J Schalkwijk; W B van den Berg; L B van de Putte; L A Joosten; L van den Bersselaar
Journal:  J Clin Invest       Date:  1985-07       Impact factor: 14.808

9.  Electrical charge of the antigen determines its localization in the mouse knee joint. Deep penetration of cationic BSA in hyaline articular cartilage.

Authors:  W B van den Berg; L B van de Putte
Journal:  Am J Pathol       Date:  1985-11       Impact factor: 4.307

10.  Enzyme histochemical reactions in unfixed and undecalcified cryostat sections of mouse knee joints with special reference to arthritic lesions.

Authors:  C J Van Noorden; I M Vogels
Journal:  Histochemistry       Date:  1986
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