Interleukin-2 production and bone-resorption activity in vitro by unstimulated lymphocytes extracted from chronically-inflamed human periodontal tissues.

Lymphocytes isolated from chronically-inflamed tissues were assessed for their ability to produce lymphokines without further stimulation in vitro. Cells were extracted from tissue obtained from 42 patients undergoing periodontal surgery. Cultures were set up in triplicate and supernatants collected after 48 h were assayed for interleukin-2 (IL-2) or bone-resorptive activity. IL-2 was assayed in the cultured supernatants from 20 patients using a previously-standardized T-cell growth assay, with maximally-stimulated peripheral blood-lymphocytes as a positive control. Bone-resorptive activity (BRA) was assessed in culture supernatants from another 22 patients using an in-vitro mouse-calvaria culture-system in which calcium release was measured with a calcium analyser. IL-2 was detected in 12 out of the 20 unstimulated cultures; BRA was detected in 14 of the 22 unstimulated cultures. There appeared to be no relationship between IL-2 production and BRA and the severity of the disease as assessed by loss of attachment. Nevertheless, it seems that most of the cells extracted from chronically-inflamed tissue were producing lymphokines which may indicate stimulation in vivo prior to cell extraction.