Literature DB >> 3876402

Purification to homogeneity of a high molecular weight human B cell growth factor; demonstration of specific binding to activated B cells; and development of a monoclonal antibody to the factor.

J L Ambrus, C H Jurgensen, E J Brown, A S Fauci.   

Abstract

High molecular weight B cell growth factor (HMW-BCGF) produced by a T cell line was purified to homogeneity and demonstrated to bind specifically to activated human B cells. A monoclonal antibody to HMW-BCGF was developed that (a) specifically inhibited the activity of HMW-BCGF in enhancing B cell proliferation, (b) specifically bound to HMW-BCGF in Western blots, (c) specifically absorbed HMW-BCGF activity from culture supernatants, and (d) specifically absorbed an internally labeled protein from T-ALL supernatant which comigrates with HMW-BCGF on sodium dodecyl sulfate-polyacrylamide gels. This antibody should help in cloning the gene for HMW-BCGF and further exploring the physiologic roles of HMW-BCGF.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3876402      PMCID： PMC2187866          DOI： 10.1084/jem.162.4.1319

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  38 in total

1.  Isolation and characterization of low and high affinity goat antibodies directed to single antigenic sites on human hemoglobin.

Authors:  A L Tan-Wilson; M Reichlin; R W Noble
Journal:  Immunochemistry       Date:  1976-11

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Isolation and structure of a human fibroblast interferon gene.

Authors:  R Derynck; J Content; E DeClercq; G Volckaert; J Tavernier; R Devos; W Fiers
Journal:  Nature       Date:  1980-06-19       Impact factor: 49.962

4.  Preparation of lymphocyte-activating factor from continuous murine macrophage cell lines.

Authors:  L B Lachman; M P Hacker; G T Blyden; R E Handschumacher
Journal:  Cell Immunol       Date:  1977-12       Impact factor: 4.868

5.  Radioiodination by use of the Bolton-Hunter and related reagents.

Authors:  J J Langone
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

Review 6.  Separate signals for the initiation of proliferation and differentiation in the b cell response to antigen.

Authors:  R W Dutton
Journal:  Transplant Rev       Date:  1975

7.  The isolation of IgG from mammalian sera with the aid of caprylic acid.

Authors:  M Steinbuch; R Audran
Journal:  Arch Biochem Biophys       Date:  1969-11       Impact factor: 4.013

8.  Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1.

Authors:  J Ohara; W E Paul
Journal:  Nature       Date:  1985 May 23-29       Impact factor: 49.962

9.  Induction of proliferation and Ig production in human B leukemic cells by anti-immunoglobulins and T cell factors.

Authors:  K Yoshizaki; T Nakagawa; T Kaieda; A Muraguchi; Y Yamamura; T Kishimoto
Journal:  J Immunol       Date:  1982-03       Impact factor: 5.422

10.  Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition.

Authors:  J W Kappler; B Skidmore; J White; P Marrack
Journal:  J Exp Med       Date:  1981-05-01       Impact factor: 14.307
View more
  22 in total

1.  Activity of soluble factors produced by Epstein-Barr virus-transformed human B lymphocytes on different cell lines.

Authors:  C Garzelli; A Bazzichi
Journal:  Experientia       Date:  1991-07-15

Review 2.  A primer on cytokines: sources, receptors, effects, and inducers.

Authors:  J H Curfs; J F Meis; J A Hoogkamp-Korstanje
Journal:  Clin Microbiol Rev       Date:  1997-10       Impact factor: 26.132

3.  Antigen-independent regulation of cytoplasmic calcium in B cells with a 12-kDa B-cell growth factor and anti-CD19.

Authors:  J A Ledbetter; P S Rabinovitch; C H June; C W Song; E A Clark; F M Uckun
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

4.  Spontaneously increased B cell growth factor and B cell differentiation factor activities in the synovial fluid of patients with rheumatoid arthritis.

Authors:  J Mulero; M Alvarez-Mon; L F de Villa; M Rodríguez; E Noguera; J M Martín-Santos; C Gutiérrez
Journal:  Ann Rheum Dis       Date:  1989-05       Impact factor: 19.103

5.  Response of neoplasms of B cell lineage to the proliferative effects of B cell growth factor and the phorbol ester TPA.

Authors:  M C Kinney; R D Collins; J Whitlock; J B Cousar; L B Vogler
Journal:  Am J Pathol       Date:  1989-04       Impact factor: 4.307

6.  Activation of human B cells mediated through two distinct cell surface differentiation antigens, Bp35 and Bp50.

Authors:  E A Clark; J A Ledbetter
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

Review 7.  Mechanisms of physiologic B cell responses and B cell hyperactivity in systemic lupus erythematosus.

Authors:  R H Zubler; Y P Huang; P A Miescher
Journal:  Springer Semin Immunopathol       Date:  1986

Review 8.  B-cell stimulatory factors (BSFs): molecular structure, biological function, and regulation of expression.

Authors:  T Kishimoto
Journal:  J Clin Immunol       Date:  1987-09       Impact factor: 8.317

9.  Soluble interleukin-2 receptor, interleukin-2 and interleukin-4 in sera and supernatants from patients with progressive systemic sclerosis.

Authors:  G Famularo; A Procopio; R Giacomelli; C Danese; S Sacchetti; M A Perego; A Santoni; G Tonietti
Journal:  Clin Exp Immunol       Date:  1990-09       Impact factor: 4.330

10.  Activation of resting human B cells by helper T-cell clone supernatant: characterization of a human B-cell-activating factor.

Authors:  A Diu; M L Gougeon; J L Moreau; E L Reinherz; J Thèze
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.