Literature DB >> 3867497

Detection of chloramphenicol acetyl transferase activity in transfected cells: a rapid and sensitive HPLC-based method.

S L Young, A E Jackson, D Puett, M H Melner.   

Abstract

An assay based on high-performance liquid chromatography (HPLC) has been developed for determining the expression of the acetyl coenzyme A: chloramphenicol 3-O-acetyltransferase gene in transfected cells. The assay is based on the separation of extracted, nonradioactive chloramphenicol and its acetylated derivatives by reverse-phase HPLC, using UV absorbance for detection. In addition to eliminating the need for costly [14C]chloramphenicol, this assay is as sensitive and much faster than the commonly used method involving thin-layer chromatography.

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Year:  1985        PMID： 3867497     DOI： 10.1089/dna.1985.4.469

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  5 in total

1.  Intron-mediated enhancement of heterologous gene expression in maize.

Authors:  D Mascarenhas; I J Mettler; D A Pierce; H W Lowe
Journal:  Plant Mol Biol       Date:  1990-12       Impact factor: 4.076

2.  Isolation and characterization of a small heat shock protein gene from maize.

Authors:  P S Dietrich; R A Bouchard; E S Casey; R M Sinibaldi
Journal:  Plant Physiol       Date:  1991-08       Impact factor: 8.340

3.  Non-radioactive detection of β-glucuronidase and chloramphenicol acetyltransferase activities in co-transformed protoplasts by HPLC.

Authors:  C Branca; A Ricci; A Torelli; S Amorosi; E Gaetani; C F Laureri; M Vitto; A Bolchi; M Brunelli; S Ottonello
Journal:  Plant Cell Rep       Date:  1993-05       Impact factor: 4.570

4.  Direct gene transfer into Actinidia deliciosa protoplasts: analysis of transient expression of the CAT gene using TLC autoradiography and a GC-MS-based method.

Authors:  M M Oliveira; J Barroso; M S Pais
Journal:  Plant Mol Biol       Date:  1991-08       Impact factor: 4.076

5.  Avocado fruit protoplasts: a cellular model system for ripening studies.

Authors:  F W Percival; L G Cass; K R Bozak; R E Christoffersen
Journal:  Plant Cell Rep       Date:  1991-12       Impact factor: 4.570
  5 in total

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