Literature DB >> 3866247

Scintillation proximity radioimmunoassay utilizing 125I-labeled ligands.

S Udenfriend, L D Gerber, L Brink, S Spector.   

Abstract

A unique type of radioimmunoassay is described that does not require centrifugation or separation. Microbeads containing a fluorophor are covalently linked to antibody. When an 125I-labeled antigen is added it binds to the beads and, by its proximity, the emitted short-range electrons of the 125I excite the fluorophor in the beads. The light emitted can be measured in a standard scintillation counter. Addition of unlabeled antigen from tissue extracts displaces the labeled ligand and diminishes the fluorescent signal. Application of scintillation proximity immunoassay to tissue enkephalins, serum thyroxin, and urinary morphine is described. Applications of the principle to study the kinetics of interaction between receptors and ligands are discussed.

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Year:  1985        PMID： 3866247      PMCID： PMC391498          DOI： 10.1073/pnas.82.24.8672

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  6 in total

Review 1.  General principles of radioimmunoassay.

Authors:  S A Berson; R S Yalow
Journal:  Clin Chim Acta       Date:  1968-09       Impact factor: 3.786

2.  A method for rapid, continuous monitoring of solute uptake and binding.

Authors:  S M Gruner; G Kirk; L Patel; H R Kaback
Journal:  Biochemistry       Date:  1982-06-22       Impact factor: 3.162

3.  Scintillation proximity assay (SPA)--a new method of immunoassay. Direct and inhibition mode detection with human albumin and rabbit antihuman albumin.

Authors:  H E Hart; E B Greenwald
Journal:  Mol Immunol       Date:  1979-04       Impact factor: 4.407

4.  Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family.

Authors:  M E Furth; L J Davis; B Fleurdelys; E M Scolnick
Journal:  J Virol       Date:  1982-07       Impact factor: 5.103

5.  Human placental alkaline phosphatase in liver and intestine.

Authors:  E Garattini; J Margolis; E Heimer; A Felix; S Udenfriend
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

6.  Specific polyclonal antibodies to the carboxyl terminus of [Met]enkephalin-Arg6-Gly7-Leu8.

Authors:  H W Lahm; L D Gerber; L Brink; D L Kilpatrick; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1983-09       Impact factor: 4.013
  6 in total
  19 in total

1.  Chronic psychosocial stress in tree shrews: effect of the substance P (NK1 receptor) antagonist L-760735 and clomipramine on endocrine and behavioral parameters.

Authors:  Marieke G C van der Hart; Gabriel de Biurrun; Boldizsár Czéh; Nadia M J Rupniak; Johan A den Boer; Eberhard Fuchs
Journal:  Psychopharmacology (Berl)       Date:  2005-10-14       Impact factor: 4.530

2.  Real-time, high-throughput measurements of peptide-MHC-I dissociation using a scintillation proximity assay.

Authors:  Mikkel Harndahl; Michael Rasmussen; Gustav Roder; Søren Buus
Journal:  J Immunol Methods       Date:  2010-10-31       Impact factor: 2.303

Review 3.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:  H M Davey; D B Kell
Journal:  Microbiol Rev       Date:  1996-12

4.  Diurnal variation of corticotropin-releasing factor binding sites in the rat brain and pituitary.

Authors:  E Fuchs; J C Wasmuth; G Flügge; G Huether; R Troost; J Beyer
Journal:  Cell Mol Neurobiol       Date:  1996-02       Impact factor: 5.046

5.  Functional and protein chemical characterization of the N-terminal domain of the rat corticotropin-releasing factor receptor 1.

Authors:  B A Hofmann; S Sydow; O Jahn; L van Werven; T Liepold; K Eckart; J Spiess
Journal:  Protein Sci       Date:  2001-10       Impact factor: 6.725

6.  A single amino acid serves as an affinity switch between the receptor and the binding protein of corticotropin-releasing factor: implications for the design of agonists and antagonists.

Authors:  K Eckart; O Jahn; J Radulovic; H Tezval; L van Werven; J Spiess
Journal:  Proc Natl Acad Sci U S A       Date:  2001-09-25       Impact factor: 11.205

7.  A miniaturized glucocorticoid receptor translocation assay using enzymatic fragment complementation evaluated with qHTS.

Authors:  Ping Jun Zhu; Wei Zheng; Douglas S Auld; Ajit Jadhav; Ryan Macarthur; Keith R Olson; Kun Peng; Hyna Dotimas; Christopher P Austin; James Inglese
Journal:  Comb Chem High Throughput Screen       Date:  2008-08       Impact factor: 1.339

8.  Hippocampal glucocorticoid receptor expression in the tree shrew: regulation by psychosocial conflict.

Authors:  O Jöhren; G Flügge; E Fuchs
Journal:  Cell Mol Neurobiol       Date:  1994-06       Impact factor: 5.046

9.  Chronic psychosocial stress causes apical dendritic atrophy of hippocampal CA3 pyramidal neurons in subordinate tree shrews.

Authors:  A M Magariños; B S McEwen; G Flügge; E Fuchs
Journal:  J Neurosci       Date:  1996-05-15       Impact factor: 6.167

10.  Measuring substrate binding and affinity of purified membrane transport proteins using the scintillation proximity assay.

Authors:  Daniel Harder; Dimitrios Fotiadis
Journal:  Nat Protoc       Date:  2012-08-02       Impact factor: 13.491
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