Literature DB >> 3866228

Nonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes.

R A Padgett, M M Konarska, M Aebi, H Hornig, C Weissmann, P A Sharp.   

Abstract

Mutants of the rabbit beta-globin gene lacking the natural site of branch formation in the second intervening sequence have been analyzed for in vitro splicing activity. RNAs transcribed from these mutants were spliced, via lariat formation, at a reduced rate compared to wild-type RNA. The sites of branch formation were mapped by direct RNA analysis and primer-extension analysis. The sequences at the branch sites in the three mutants examined did not conform to the previously determined consensus sequence, nor were the 5' splice sites and branch sites complementary.

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Year:  1985        PMID： 3866228      PMCID： PMC390913          DOI： 10.1073/pnas.82.24.8349

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.

Authors:  B Ruskin; A R Krainer; T Maniatis; M R Green
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

2.  Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for U1 RNA and metazoan mRNA splicing.

Authors:  C W Pikielny; J L Teem; M Rosbash
Journal:  Cell       Date:  1983-09       Impact factor: 41.582

3.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors:  D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

4.  Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.

Authors:  R A Padgett; M M Konarska; P J Grabowski; S F Hardy; P A Sharp
Journal:  Science       Date:  1984-08-31       Impact factor: 47.728

5.  A minimal intron length but no specific internal sequence is required for splicing the large rabbit beta-globin intron.

Authors:  B Wieringa; E Hofer; C Weissmann
Journal:  Cell       Date:  1984-07       Impact factor: 41.582

6.  Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit beta-globin gene.

Authors:  B Wieringa; F Meyer; J Reiser; C Weissmann
Journal:  Nature       Date:  1983-01-06       Impact factor: 49.962

7.  Splicing of adenovirus RNA in a cell-free transcription system.

Authors:  R A Padgett; S F Hardy; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

8.  Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.

Authors:  C J Langford; F J Klinz; C Donath; D Gallwitz
Journal:  Cell       Date:  1984-03       Impact factor: 41.582

9.  DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.

Authors:  P Dierks; A van Ooyen; N Mantei; C Weissmann
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205

10.  Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.

Authors:  E B Keller; W A Noon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205
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  42 in total

1.  The mutational spectrum of single base-pair substitutions in mRNA splice junctions of human genes: causes and consequences.

Authors:  M Krawczak; J Reiss; D N Cooper
Journal:  Hum Genet       Date:  1992 Sep-Oct       Impact factor: 4.132

2.  Mutational analysis of the U12-dependent branch site consensus sequence.

Authors:  Jay E Brock; Rosemary C Dietrich; Richard A Padgett
Journal:  RNA       Date:  2008-09-29       Impact factor: 4.942

3.  Splicing of group II introns in spinach chloroplasts (in vivo): analysis of lariat formation.

Authors:  J K Kim; M J Hollingsworth
Journal:  Curr Genet       Date:  1993-02       Impact factor: 3.886

4.  Interplay between U2 snRNP and 3' splice factor(s) for branch point selection on human beta-globin pre-mRNA.

Authors:  C Alibert; J Tazi; J Temsamani; P Jeanteur; C Brunel; G Cathala
Journal:  Nucleic Acids Res       Date:  1990-01-25       Impact factor: 16.971

5.  Control of retroviral RNA splicing through maintenance of suboptimal processing signals.

Authors:  R A Katz; A M Skalka
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

6.  Precise branch point mapping and quantification of splicing intermediates.

Authors:  J Vogel; W R Hess; T Börner
Journal:  Nucleic Acids Res       Date:  1997-05-15       Impact factor: 16.971

7.  Factor interactions with the simian virus 40 early pre-mRNA influence branch site selection and alternative splicing.

Authors:  J C Noble; H Ge; M Chaudhuri; J L Manley
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

8.  The 216-nucleotide intron of the E1A pre-mRNA contains a hairpin structure that permits utilization of unusually distant branch acceptors.

Authors:  K Chebli; R Gattoni; P Schmitt; G Hildwein; J Stevenin
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

9.  The quantitation and distribution of splicing intermediates in HeLa cells and adenovirus RNAs.

Authors:  J D Reilly; J C Wallace; M Edmonds
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

10.  Unusual branch point selection involved in splicing of the alternatively processed Calcitonin/CGRP-I pre-mRNA.

Authors:  G J Adema; R A Bovenberg; H S Jansz; P D Baas
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971
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