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Literature DB >> 3865893

Isolation of a rat immune response gene identical to an alleged mouse a class II beta-chain pseudogene.

Abstract

A human HLA-DQ beta-chain cDNA was used as a probe to identify and isolate a rat major histocompatibility antigen beta-chain gene from a genomic library constructed in the vector lambda Charon 28 using Wistar rat DNA (RT1u). The isolated exon of the rat gene (RT1.B beta 2) encoding a beta-chain second domain was found to share 93% nucleotide homology with a mouse A beta 2 exon. Although the genomic organization of this gene is consistent with the hypothesis that it represents a pseudogene, the remarkable preservation of a specific sequence favors the view that this class II antigen beta-chain gene has retained its coding function.

Entities: Gene Species

Mesh：

Substances：

Year: 1985 PMID： 3865893 DOI： 10.1007/bf00430308

Source DB: PubMed Journal: Immunogenetics ISSN： 0093-7711 Impact factor: 2.846

26 in total

1. In vitro packaging of lambda and cosmid DNA.

Authors: B Hohn
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

2. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Authors: A Böyum
Journal: Scand J Clin Lab Invest Suppl Date: 1968

3. Open reading frame sequencing and structure-based alignment of polypeptides encoded by RT1-Bb, RT1-Ba, RT1-Db, and RT1-Da alleles.

Authors: Ruth A Ettinger; Antonis K Moustakas; Suzanne D Lobaton
Journal: Immunogenetics Date: 2004-10-23 Impact factor: 2.846

4. The alpha chain gene of H-2O has an unexpected location in the major histocompatibility complex.

Authors: L Karlsson; P A Peterson
Journal: J Exp Med Date: 1992-08-01 Impact factor: 14.307

4 in total

Isolation of a rat immune response gene identical to an alleged mouse a class II beta-chain pseudogene.

1. In vitro packaging of lambda and cosmid DNA.

2. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.

Review 3. Role of MHC gene products in immune regulation.

4. Construction of coliphage lambda Charon vectors with BamHI cloning sites.

5. A molecular map of the immune response region from the major histocompatibility complex of the mouse.

6. Isolation of a cDNA clone coding for an SB beta-chain.

7. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.

8. The isolation of structural genes from libraries of eucaryotic DNA.

9. Restriction fragment polymorphisms in the major histocompatibility complex of diabetic BB rats.

10. Mutations and selection in the generation of class II histocompatibility antigen polymorphism.

1. Polymorphism and mapping of the class II genes in the rat: RT1.B, RT1.D, and RT1.H, a new DP-like region.

2. Cloning and analysis of a new rat major histocompatibility complex class II gene, RT1.DOa.

3. Open reading frame sequencing and structure-based alignment of polypeptides encoded by RT1-Bb, RT1-Ba, RT1-Db, and RT1-Da alleles.

4. The alpha chain gene of H-2O has an unexpected location in the major histocompatibility complex.