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Literature DB >> 3856231

Colony formation by primitive hemopoietic progenitor cells in serum-free medium.

Abstract

A serum-free medium has been developed for clonal growth of murine hemopoietic progenitor cells. In this medium, the number of nonerythroid colonies induced by factors produced by cloned T lymphocytes was 90 +/- 10% of the number generated in serum-containing medium. Erythroid colony number in serum-free cultures containing the T-cell factors but no exogenous erythropoietin was significantly higher than that in cultures with serum, and the cloning efficiency was independent of cell concentration. Further addition of erythropoietin increased erythroid colony number approximately equal to 4-fold. Pure erythropoietin alone stimulated erythroid colony formation, but the cloning efficiency was highly dependent on cell concentration. Analysis of individual colonies generated in serum-free cultures containing the T-cell factors indicated that some contained cells of several hemopoietic lineages, demonstrating that multipotential progenitors can give rise to colonies in this system.

Entities: Gene Species

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Year: 1985 PMID： 3856231 PMCID： PMC397129 DOI： 10.1073/pnas.82.3.775

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.

Authors: E R Stanley; P M Heard
Journal: J Biol Chem Date: 1977-06-25 Impact factor: 5.157

2. Control of protein synthesis by hemin. Evidence that the hemin-controlled translational repressor inhibits formation of 80 S initiation complexes from 48 S intermediate initiation complexes.

Authors: M Gross
Journal: J Biol Chem Date: 1979-04-10 Impact factor: 5.157

3. Physical separation of hemopoietic stem cells from cells forming colonies in culture.

Authors: R G Worton; E A McCulloch; J E Till
Journal: J Cell Physiol Date: 1969-10 Impact factor: 6.384

4. Purification and properties of colony-stimulating factor from mouse lung-conditioned medium.

Authors: A W Burgess; J Camakaris; D Metcalf
Journal: J Biol Chem Date: 1977-03-25 Impact factor: 5.157

5. Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol.

Authors: N N Iscove; L J Guilbert; C Weyman
Journal: Exp Cell Res Date: 1980-03 Impact factor: 3.905

9. Interleukin 3 perfusion in W/Wv mice allows the development of macroscopic hematopoietic spleen colonies and restores cutaneous mast cell number.

Authors: C Ody; V Kindler; P Vassalli
Journal: J Exp Med Date: 1990-07-01 Impact factor: 14.307

10. Serum-free Erythroid Differentiation for Efficient Genetic Modification and High-Level Adult Hemoglobin Production.

Authors: Naoya Uchida; Selami Demirci; Juan J Haro-Mora; Atsushi Fujita; Lydia N Raines; Matthew M Hsieh; John F Tisdale
Journal: Mol Ther Methods Clin Dev Date: 2018-03-22 Impact factor: 6.698

Colony formation by primitive hemopoietic progenitor cells in serum-free medium.

1. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.

2. Control of protein synthesis by hemin. Evidence that the hemin-controlled translational repressor inhibits formation of 80 S initiation complexes from 48 S intermediate initiation complexes.

3. Physical separation of hemopoietic stem cells from cells forming colonies in culture.

4. Purification and properties of colony-stimulating factor from mouse lung-conditioned medium.

5. Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol.

6. Purification of human erythropoietin.

7. Induction of erythropoietin responsiveness in vitro by a distinct population of bone marrow cells.

8. Enhancement of erythroid colony growth in culture by hemin.

9. The role of hemin in the regulation of heme synthesis by fetal mouse liver erythroblasts in culture.

10. Improving techniques for clonogenic assays.

1. Clonogenic assay of leukemic cells in serum-free culture.

2. Stimulation of hematopoiesis in vivo by recombinant bacterial murine interleukin 3.

3. Effect of human plasmalipoproteins on erythropoietic progenitor cells in serum-free cultures.

4. Humanized culture medium for clinical expansion of human erythroblasts.

5. Lymphoma cell culture under serum-free conditions.

6. Clonogenic growth of acute non-lymphocytic leukemia cells in serum-free medium.

7. Defined, serum-free culture conditions for the GM-micro-clonogenic assay using agar-capillaries.

8. Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum.

9. Interleukin 3 perfusion in W/Wv mice allows the development of macroscopic hematopoietic spleen colonies and restores cutaneous mast cell number.

10. Serum-free Erythroid Differentiation for Efficient Genetic Modification and High-Level Adult Hemoglobin Production.