Literature DB >> 385049

Isolation and characterization of fourteen ribosomal proteins from small subunits of yeast.

K Higo, E Otaka.   

Abstract

A method for preparation of a large amount of ribosomal subunits from Saccharomyces cerevisiae by a Ti-15 zonal rotor is described. The proteins of the small subunits (ca. 50 000 A260 units) were separated into 22 fractions by chromatography on carboxymethylcellulose columns. Fourteen proteins were then purified from the ten chromatographic fractions by filtration through Sephadex G-100 or Sephacryl S--200. The isolated proteins are YP 6, YP 7, YP 9, YP 12, YP 14', YP 14'', YP 28, YP 38, YP 45, YP 50, YP 52, YP 58, YP 63, and YP 70. The molecular weight and amino acid compositions of these proteins are presented.

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Year:  1979        PMID： 385049     DOI： 10.1021/bi00586a024

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  8 in total

1.  Ribosomal proteins of Thermomyces lanuginosus--characterisation by two-dimensional gel electrophoresis and differential disassembly.

Authors:  J Wu; D R Beniac; G Harauz
Journal:  Mol Cell Biochem       Date:  1995-02-09       Impact factor: 3.396

2.  Sequence and functional similarity between a yeast ribosomal protein and the Escherichia coli S5 ram protein.

Authors:  J A All-Robyn; N Brown; E Otaka; S W Liebman
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

3.  A rapid and preparative method for the separation of yeast ribosomal proteins by using high-performance liquid chromatography.

Authors:  G J Threadgill; R C Conrad; M Cannon; G R Craven
Journal:  Biochem J       Date:  1987-06-15       Impact factor: 3.857

4.  Yeast ribosomal proteins: XII. YS11 of Saccharomyces cerevisiae is a homologue to E. coli S4 according to the gene analysis.

Authors:  K Mizuta; T Hashimoto; K Suzuki; E Otaka
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

5.  Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes.

Authors:  E Otaka; K Higo; T Itoh
Journal:  Mol Gen Genet       Date:  1984

6.  Yeast ribosomal protein S33 is encoded by an unsplit gene.

Authors:  R J Leer; M M van Raamsdonk-Duin; P J Schoppink; M T Cornelissen; L H Cohen; W H Mager; R J Planta
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

7.  Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe.

Authors:  E Otaka; K Higo; T Itoh
Journal:  Mol Gen Genet       Date:  1983

8.  Genetic and biochemical characterization of antisuppressor mutants in the yeast Saccharomyces cerevisiae.

Authors:  J Ishiguro
Journal:  Curr Genet       Date:  1981-12       Impact factor: 3.886
  8 in total

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