| Literature DB >> 384995 |
Abstract
ATP consumption by arginyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophilus has been investigated by the firefly luciferin--luciferase assay. Arginyl-tRNA synthetase from E. coli utilizes ATP only for aminocylation of tRNA with a 1:1 stoicheiometry. In contrast, we have shown an adenosine triphosphatase activity of arginyl-tRNA synthetase from B. stearothermophilus in the absence of tRNAArg. Dowex chromatography revealed the formation of ADP by the thermophile enzyme; under aminoacylation conditions, AMP was also formed in amounts stoicheiometric with arginyl-tRNA formation.Entities:
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Year: 1979 PMID: 384995 PMCID: PMC1186638 DOI: 10.1042/bj1790407
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857