Literature DB >> 384995

Adenosine triphosphate consumption by bacterial arginyl-transfer ribonucleic acid synthetases.

J M Godeau, J Charlier.   

Abstract

ATP consumption by arginyl-tRNA synthetases from Escherichia coli and Bacillus stearothermophilus has been investigated by the firefly luciferin--luciferase assay. Arginyl-tRNA synthetase from E. coli utilizes ATP only for aminocylation of tRNA with a 1:1 stoicheiometry. In contrast, we have shown an adenosine triphosphatase activity of arginyl-tRNA synthetase from B. stearothermophilus in the absence of tRNAArg. Dowex chromatography revealed the formation of ADP by the thermophile enzyme; under aminoacylation conditions, AMP was also formed in amounts stoicheiometric with arginyl-tRNA formation.

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Year:  1979        PMID: 384995      PMCID: PMC1186638          DOI: 10.1042/bj1790407

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  10 in total

1.  MECHANISM OF AMINOACYL RNA SYNTHESIS: STUDIES WITH ISOLATED AMINOACYL ADENYLATE COMPLEXES OF ISOLEUCYL RNA SYNTHETASE.

Authors:  A T NORRIS; P BERG
Journal:  Proc Natl Acad Sci U S A       Date:  1964-08       Impact factor: 11.205

2.  THE PREPARATION OF AMINO ACID-TRANSFER RIBONUCLEIC ACID FROM ESCHERICHIA COLI BY DIRECT PHENOL EXTRACTION OF INTACT CELLS.

Authors:  L H BRUBAKER; D J MCCORQUODALE
Journal:  Biochim Biophys Acta       Date:  1963-09-17

3.  Purification of valine transfer ribonucleic acid by combined chromatographic and chemical procedures.

Authors:  M L STEPHENSON; P C ZAMECNIK
Journal:  Proc Natl Acad Sci U S A       Date:  1961-10-15       Impact factor: 11.205

4.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

5.  Arginyl-tRNA synthetase from Bacillus stearothermophilus: subunit structure of enzyme.

Authors:  J M Godeau
Journal:  FEBS Lett       Date:  1976-02-15       Impact factor: 4.124

6.  Affinity elution as a purification method for aminoacyl-tRNA synthetases.

Authors:  F von der Haar
Journal:  Eur J Biochem       Date:  1973-04-02

7.  Isoleucyl-transfer ribonucleic acid synthetase from Bacillus stearothermophilus. I. Properties of the enzyme.

Authors:  J Charlier; H Grosjean
Journal:  Eur J Biochem       Date:  1972-01-31

8.  A general procedure for the isolation of specific transfer ribonucleic acids.

Authors:  I Gillam; D Blew; R C Warrington; M von Tigerstrom; G M Tener
Journal:  Biochemistry       Date:  1968-10       Impact factor: 3.162

9.  On the properties and utility of a membrane filter assay in the study of isoleucyl-tRNA synthetase.

Authors:  M Yarus; P Berg
Journal:  Anal Biochem       Date:  1970-06       Impact factor: 3.365

10.  Arginyl-tRNA synthetase from Escherichia coli. Influence of arginine biosynthetic precursors on the charging of arginine-acceptor tRNA with [14C]arginine.

Authors:  J Charlier; E Gerlo
Journal:  Eur J Biochem       Date:  1976-11-01
  10 in total
  3 in total

1.  In vivo selection of lethal mutations reveals two functional domains in arginyl-tRNA synthetase.

Authors:  R Geslain; F Martin; B Delagoutte; J Cavarelli; J Gangloff; G Eriani
Journal:  RNA       Date:  2000-03       Impact factor: 4.942

2.  Arginyl-tRNA synthetase with signature sequence KMSK from Bacillus stearothermophilus.

Authors:  Juan Li; Yong-Neng Yao; Mo-Fang Liu; En-Duo Wang
Journal:  Biochem J       Date:  2003-12-15       Impact factor: 3.857

3.  Identification of potent inhibitors of the Trypanosoma brucei methionyl-tRNA synthetase via high-throughput orthogonal screening.

Authors:  Laura Pedró-Rosa; Frederick S Buckner; Ranae M Ranade; Christina Eberhart; Franck Madoux; J Robert Gillespie; Cho Yeow Koh; Steven Brown; Jacqueline Lohse; Christophe L M Verlinde; Erkang Fan; Thomas Bannister; Louis Scampavia; Wim G J Hol; Timothy Spicer; Peter Hodder
Journal:  J Biomol Screen       Date:  2014-08-27
  3 in total

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