Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Transferrin in isolated cells from rat duodenum and jejunum.

Literature DB >> 3848337

Transferrin in isolated cells from rat duodenum and jejunum.

K Osterloh, K Schümann, C Ehtechami, W Forth.

Abstract

Mucosal transferrin was determined as transferrin-like immunoreactivity (TLIR) by means of a 2-site immunoradiometric assay (IRMA). Scraped-off mucosal tissue as well as isolated mucosal cells from the duodenum and jejunum of normal and iron-deficient rats before and after a washing procedure were examined. In iron-deficient rats there was about twice as much TLIR in scraped-off mucosal tissue as in the untreated animals. In the duodenum and jejunum of normal and iron-deficient rats, TLIR contents of the isolated cells in the magnitude of 320-510 ng/mg dry weight were found. Washing isolated cells three times in ice-cold Hank's solution resulted in a nearly tenfold decrease of TLIR content in all groups. In contrast the cells' RNA content remained unchanged.

Entities: Chemical Gene Species

Mesh：

Substances：
Transferrin
Iron

Year: 1985 PMID： 3848337 DOI： 10.1007/bf00320599

Source DB: PubMed Journal: Blut ISSN： 0006-5242

14 in total

1. A new class of uncoupling agents--carbonyl cyanide phenylhydrazones.

Authors: P G HEYTLER; W W PRICHARD
Journal: Biochem Biophys Res Commun Date: 1962-05-04 Impact factor: 3.575

2. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation.

Authors: M M Weiser
Journal: J Biol Chem Date: 1973-04-10 Impact factor: 5.157

3. [Measurements of metabolism and lysis during the incubation of epithelial cells isolated from small intestine of the rat in presence of various surface-active agents (author's transl)].

Authors: M T Mitjavila; S Mitjavila; R Derache
Journal: Toxicology Date: 1973-09 Impact factor: 4.221

4. Improved methods for determination of RNA and DNA.

Authors: D W Hatcher; G Goldstein
Journal: Anal Biochem Date: 1969-10-01 Impact factor: 3.365

5. [Method for induction of iron deficiency in rats].

Authors: W Forth; H Andres
Journal: Arzneimittelforschung Date: 1969-03

6. Localization of transferrin in rat duodenal mucosa by immunoperoxidase technique.

Authors: K Isobe; Y Isobe
Journal: Nihon Ketsueki Gakkai Zasshi Date: 1983-07

7. Determination of transferrin-like immunoreactivity in the mucosal homogenate of the duodenum, jejunum, and ileum of normal and iron deficient rats.

Authors: K Osterloh; W Forth
Journal: Blut Date: 1981-10

8. Distribution of transferrin, ferritin, and lactoferrin in human tissues.

Authors: D Y Mason; C R Taylor
Journal: J Clin Pathol Date: 1978-04 Impact factor: 3.411

9. Chronic experimental iron losses in rats not leading to overt iron deficiency: a model for the regulation of the whole-body iron balance in an iron-replete condition.

Authors: M Bhargava; E E Gabbe
Journal: J Nutr Date: 1984-06 Impact factor: 4.798

10. The kinetics and mechanism of iron(3) exchange between chelates and transferrin. IV. The reaction of transferrin with iron(3) nitrilotriacetate.

Authors: G W Bates; J Wernicke
Journal: J Biol Chem Date: 1971-06-10 Impact factor: 5.157

5 in total

Transferrin in isolated cells from rat duodenum and jejunum.

1. A new class of uncoupling agents--carbonyl cyanide phenylhydrazones.

2. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation.

3. [Measurements of metabolism and lysis during the incubation of epithelial cells isolated from small intestine of the rat in presence of various surface-active agents (author's transl)].

4. Improved methods for determination of RNA and DNA.

5. [Method for induction of iron deficiency in rats].

6. Localization of transferrin in rat duodenal mucosa by immunoperoxidase technique.

7. Determination of transferrin-like immunoreactivity in the mucosal homogenate of the duodenum, jejunum, and ileum of normal and iron deficient rats.

8. Distribution of transferrin, ferritin, and lactoferrin in human tissues.

9. Chronic experimental iron losses in rats not leading to overt iron deficiency: a model for the regulation of the whole-body iron balance in an iron-replete condition.

10. The kinetics and mechanism of iron(3) exchange between chelates and transferrin. IV. The reaction of transferrin with iron(3) nitrilotriacetate.

1. Duodenal iron proteins in idiopathic hemochromatosis.

2. Intestinal iron absorption and mucosal transferrin in rats subjected to hypoxia.

Review 3. [The role of iron as a deficient element].

4. Independence of in vitro iron absorption from mucosal transferrin content in rat jejunal and ileal segments.

5. Supplemental Microalgal Iron Helps Replete Blood Hemoglobin in Moderately Anemic Mice Fed a Rice-Based Diet.