Literature DB >> 384367

Characterization of an improved in vitro DNA replication system for Escherichia coli plasmids.

S E Conrad, J L Campbell.   

Abstract

A modified in vitro replication system has been characterized and used to catalogue the host proteins required for the replication of plasmid RSF1030. These extracts differ from systems described previously in that endogenous DNA is removed. Replication in vitro therefore requires an exogenouos RSF1030. Synthesis in the in vitro system faithfully mimics in vivo replication with respect to the products synthesized, effects of specific inhibitors, and requirements for RNA polymerase and DNA polymerase I. In addition, we find that proteins encoded by dnaB, dnaC, dnaG, dnaI, dnaP and polC (DNA polymerase III), are required for in vitro plasmid synthesis. The product of dnaA is not required. Extracts prepared from E. coli mutants deficient in in vitro replication can be complemented by addition of purified proteins or of extracts carrying the wild type protein.

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Year:  1979        PMID： 384367      PMCID： PMC327934          DOI： 10.1093/nar/6.10.3289

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA.

Authors:  R WEIL; J VINOGRAD
Journal:  Proc Natl Acad Sci U S A       Date:  1963-10       Impact factor: 11.205

2.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

3.  Replication of small plasmids in extracts of Escherichia coli: involvement of the dnaB and dnaC protein in the replication of early replicative intermediates.

Authors:  W L Staudenbauer; E Lanka; H Schuster
Journal:  Mol Gen Genet       Date:  1978-07-04

Review 4.  DNA replication proteins of Escherichia coli.

Authors:  S H Wickner
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

5.  Origin and direction of DNA replication of plasmid RSF1030.

Authors:  S E Conrad; M Wold; J L Campbell
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

6.  Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins.

Authors:  D J Donoghue; P A Sharp
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

7.  Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.

Authors:  J Collins; P Williams; D R Helinski
Journal:  Mol Gen Genet       Date:  1975

8.  REPLICAtion of small plasmids in extracts of Escherichia coli: requirement for both DNA polymerases I and II.

Authors:  W L Staudenbauer
Journal:  Mol Gen Genet       Date:  1976-12-08

9.  Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; T Itoh; J I Tomizawa
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

10.  Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.

Authors:  M Kahn; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205
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  17 in total

1.  Sequence-specific and polarized replication termination in vitro: complementation of extracts of tus- Escherichia coli by purified Ter protein and analysis of termination intermediates.

Authors:  T MacAllister; G S Khatri; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

2.  Characterization and complementation of pMB1 copy number mutant: effect of RNA I gene dosage on plasmid copy number and incompatibility.

Authors:  D R Moser; J L Campbell
Journal:  J Bacteriol       Date:  1983-05       Impact factor: 3.490

3.  Discriminatory function of ribonuclease H in the selective initiation of plasmid DNA replication.

Authors:  G Hillenbrand; W L Staudenbauer
Journal:  Nucleic Acids Res       Date:  1982-02-11       Impact factor: 16.971

4.  Enzymatic replication of the origin of the Escherichia coli chromosome.

Authors:  R S Fuller; J M Kaguni; A Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

5.  Accumulation of ColE1 early replicative intermediates catalyzed by extracts of Escherichia coli dnaG mutant strains.

Authors:  L Fouser; R E Bird
Journal:  J Bacteriol       Date:  1983-06       Impact factor: 3.490

6.  Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

Authors:  J Germino; J G Gray; H Charbonneau; T Vanaman; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

7.  The involvement of host replication proteins and of specific origin sequences in the in vitro replication of miniplasmid R1 DNA.

Authors:  S Ortega; E Lanka; R Diaz
Journal:  Nucleic Acids Res       Date:  1986-06-25       Impact factor: 16.971

8.  Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.

Authors:  M Noyer-Weidner; R Diaz; L Reiners
Journal:  Mol Gen Genet       Date:  1986-12

9.  Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.

Authors:  R Diaz; W L Staudenbauer
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

10.  The heat-shock DnaK protein is required for plasmid R1 replication and it is dispensable for plasmid ColE1 replication.

Authors:  R Giraldo-Suárez; E Fernández-Tresguerres; R Díaz-Orejas; A Malki; M Kohiyama
Journal:  Nucleic Acids Res       Date:  1993-11-25       Impact factor: 16.971
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